Description

n-(4-Azido-2-Nitrophenyl)-5-Hydroxytryptamine CAS NO 94452-30-9 is a specialized, high-purity biochemical reagent featuring a unique azido-nitroaryl functional group conjugated to a hydroxytryptamine moiety. This compound is critically important as a photoactivatable crosslinking agent and a versatile chemical probe for advanced life science research. It is primarily needed by researchers and developers in pharmaceutical R&D, neurochemistry, and bioconjugation for studying receptor-ligand interactions and protein labeling.

Application

• Photoaffinity Labeling: Used to irreversibly tag and study serotonin receptors and other biomolecular targets upon UV light activation.
• Bioconjugation & Probe Synthesis: Serves as a key intermediate for creating fluorescent, radioactive, or affinity-tagged probes for cellular imaging and assays.
• Neuropharmacology Research: Employed in the investigation of serotonin (5-HT) receptor structure, function, and signaling pathways.
• Chemical Biology Tool: Utilized for mapping binding sites and identifying unknown protein targets in complex biological systems.
• Proteomics Studies: Applied in activity-based protein profiling (ABPP) to discover and characterize enzymes and their inhibitors.
• Drug Discovery Platforms: Integrated into high-throughput screening assays to identify and validate novel therapeutic candidates.

Basic Information

Product Name	n-(4-Azido-2-Nitrophenyl)-5-Hydroxytryptamine
CAS No.	94452-30-9
Molecular Formula	C16H14N6O3
Molecular Weight	338.32 g/mol
Synonyms	5-Hydroxytryptamine, N-[4-azido-2-nitrophenyl]-; 5-HT-ANP; ANP-5-HT; N-(4-Azido-2-nitrophenyl)serotonin; Serotonin azidonitrophenyl derivative; Azidonitrophenyl hydroxytryptamine; Photoactivatable serotonin analog; 4-Azido-2-nitrophenyl-5-hydroxytryptamine
EINECS	Contact for details

Quality Control

Our n-(4-Azido-2-Nitrophenyl)-5-Hydroxytryptamine is manufactured under controlled conditions to ensure high batch-to-batch consistency and purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and spectroscopic techniques (NMR, MS) for structural confirmation. A detailed Certificate of Analysis (COA) documenting identity, purity, and related substances is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is strictly light-sensitive (protect from all light exposure) and should be handled under subdued lighting conditions. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	≤ 5.0% total
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.