Description

H-Tyr-Amc Tfa is a specialized protected amino acid derivative, specifically a tyrosine-based fluorogenic substrate. This compound is critical for biochemical research and pharmaceutical development, serving as a key building block or enzymatic substrate. It is primarily utilized by researchers and manufacturers in the fields of protease enzyme studies, peptide synthesis, and high-throughput screening assays. The product is supplied with a high level of purity to ensure reliable and reproducible experimental results.

Application

• Protease Enzyme Assays: Acts as a sensitive fluorogenic substrate for studying the activity and kinetics of various proteolytic enzymes, including caspases and other serine or cysteine proteases.
• Biochemical Research: Used as a key reagent in fundamental research to investigate enzyme mechanisms, inhibition, and cellular signaling pathways.
• Drug Discovery & High-Throughput Screening (HTS): Employed in pharmaceutical R&D for screening compound libraries to identify potential protease inhibitors as drug candidates.
• Peptide Synthesis: Serves as a protected tyrosine derivative (H-Tyr) in solid-phase or solution-phase peptide synthesis for constructing complex peptide sequences.
• Diagnostic Kit Development: Incorporated into the development of diagnostic assays and kits for detecting specific protease activities associated with diseases.

Basic Information

Product Name	H-Tyr-Amc Tfa
CAS No.	94099-57-7
Molecular Formula	C₂₁H₂₀F₃N₃O₆
Molecular Weight	467.40 g/mol
Synonyms	L-Tyrosine-7-amido-4-methylcoumarin trifluoroacetate salt; N-[(tert-Butoxy)carbonyl]-L-tyrosine 7-amido-4-methylcoumarin TFA salt; H-Tyr-AMC; Boc-Tyr-AMC TFA; Tyr-AMC; AMC substrate for tyrosine proteases; L-Tyrosine AMC derivative; N-Boc-L-tyrosine 7-amido-4-methylcoumarin
EINECS	Contact for details

Quality Control

Every batch of H-Tyr-Amc Tfa is subjected to rigorous analytical testing to ensure identity, purity, and consistency. Our quality assurance protocols are designed to meet the stringent requirements of research and development. A comprehensive Certificate of Analysis (COA) detailing purity (typically by HPLC), identity confirmation (by NMR and/or IR), and other critical parameters is provided with each shipment to guarantee reliable performance in your applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive) and should be kept under desiccant in its original container. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.