Description

2,3,5-Triphenyl-2H-Tetrazolium Chloride Monohydrochloride is a vital tetrazolium salt widely used as a redox indicator and a critical reagent in biochemical assays. Its primary value lies in its ability to be reduced to a brightly colored, water-insoluble formazan dye, making it an essential tool for detecting cellular metabolic activity. This compound is indispensable for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, particularly for applications like cell viability testing (MTT assays) and microbiology.

Application

• Cell Viability and Proliferation Assays (MTT Assay): The most prominent application, where it is reduced by mitochondrial dehydrogenases in living cells to a purple formazan product, providing a quantitative measure of metabolic activity.
• Microbiological Testing: Used as an indicator in culture media to detect microbial growth and metabolic activity, including applications in sterility testing and antimicrobial susceptibility.
• Biochemical Research: Serves as an electron acceptor in dehydrogenase enzyme assays and other redox-based biochemical studies.
• Histology and Staining: Employed in tissue staining to localize sites of dehydrogenase enzyme activity.
• Seed Viability Testing: Applied in agricultural sciences to assess the germination potential and metabolic state of seeds.
• Pharmaceutical Quality Control: Utilized in potency tests and sterility checks for injectable products and other sterile pharmaceuticals.

Basic Information

Product Name	2,3,5-Triphenyl-2H-Tetrazolium Chloride Monohydrochloride
CAS No.	93919-46-1
Molecular Formula	C19H16ClN4•HCl
Molecular Weight	377.27 g/mol (monohydrochloride salt)
Synonyms	TPTZ Monohydrochloride; TTC Monohydrochloride; Triphenyltetrazolium Chloride Hydrochloride; 2,3,5-Triphenyltetrazolium Chloride HCl; Tetrazolium Red Chloride; MTT Tetrazolium Salt (related); 2,3,5-Triphenyl-2H-tetrazolium chloride, hydrochloride; 2,3,5-Triphenyl-2H-tetrazol-3-ium chloride hydrochloride
EINECS	Contact for details

Quality Control

Our 2,3,5-Triphenyl-2H-Tetrazolium Chloride Monohydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR spectroscopy, and verification of performance in standard biochemical assays. We provide full traceability and Certificates of Analysis (COA) are available for all shipments to ensure compliance with your research and production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent absorption of moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	Yellow to pale yellow crystalline powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Biological Activity (MTT Assay)	Conforms to reference standard performance

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.