Description

5-Bromo-6-Chloro-3-Indolyl-β-D-Galactoside is a chromogenic substrate widely used in molecular biology and biochemistry for the detection of β-galactosidase enzyme activity. This compound is critical for applications such as blue-white screening in recombinant DNA technology and histochemical staining. It is an essential reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries requiring precise enzymatic assays.

Application

• Blue-White Screening in Cloning: As a key substrate for identifying recombinant bacterial colonies containing plasmids with inserted DNA fragments via β-galactosidase activity.
• Histochemistry & Cytochemistry: Used for the localization and visualization of β-galactosidase expression in tissue sections and cell cultures.
• Enzyme-Linked Reporter Assays: Employed in various enzymatic assays where β-galactosidase is used as a reporter gene.
• Microbiological Research: For studying gene expression and regulation in microbial systems.
• Diagnostic Kit Manufacturing: A component in the production of kits for molecular diagnostics and research.
• Biochemical Research: For kinetic studies and characterization of β-galactosidase enzymes from different sources.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indolyl-β-D-Galactoside
CAS No.	93863-88-8
Molecular Formula	C14H15BrClNO6
Molecular Weight	408.63 g/mol
Synonyms	X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside) analog; 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside; BCIG; 6-Chloro-5-bromo-3-indolyl-β-D-galactoside; Magenta-Gal; 93863-88-8; 5-Br-6-Cl-3-Indolyl-β-D-Gal
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indolyl-β-D-Galactoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant quality management standards to support our global clientele in the life sciences sector.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Melting Point	Contact for details
Heavy Metals	< 20 ppm
Performance (Enzymatic Assay)	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.