Description

4-Nitrophenyl2-Acetamido-2-Deoxy-3-o-[2-o-(A-L-Fucopyranosyl)-B-D-Galactopyranosyl]-B-D-Glucopyranoside is a specialized synthetic trisaccharide derivative, serving as a chromogenic substrate for the detection and study of specific glycosidase enzymes. Its core value lies in enabling precise biochemical research and diagnostic assay development through its specific cleavage by α-L-fucosidase and related enzymes. This compound is essential for researchers and manufacturers in the fields of biochemistry, enzymology, and in vitro diagnostic (IVD) kit production, where reliable, high-purity substrates are critical for accurate results.

Application

• Enzyme Activity Assays: Primary substrate for measuring α-L-fucosidase activity in research and clinical diagnostics.
• Diagnostic Reagent Manufacturing: Key component in the formulation of kits for detecting genetic disorders like fucosidosis or for cancer marker studies.
• Biochemical Research: Used to investigate glycosidase specificity, enzyme kinetics, and carbohydrate metabolism pathways.
• Glycobiology Studies: A tool for probing the structure and function of complex carbohydrates and glycoconjugates.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential glycosidase inhibitors for drug discovery.
• Quality Control in Bioprocessing: Used to monitor glycosidase activity during the production and purification of biopharmaceuticals.

Basic Information

Product Name	4-Nitrophenyl2-Acetamido-2-Deoxy-3-o-[2-o-(A-L-Fucopyranosyl)-B-D-Galactopyranosyl]-B-D-Glucopyranoside
CAS No.	93496-53-8
Molecular Formula	C32H46N2O20
Molecular Weight	778.71 g/mol
Synonyms	4-Nitrophenyl α-L-fucopyranosyl-(1→2)-β-D-galactopyranosyl-(1→3)-2-acetamido-2-deoxy-β-D-glucopyranoside; p-Nitrophenyl-2-acetamido-2-deoxy-3-O-[2-O-(α-L-fucopyranosyl)-β-D-galactopyranosyl]-β-D-glucopyranoside; Fucosyl-α(1-2)-galactosyl-β(1-3)-N-acetylglucosaminide 4-nitrophenyl derivative; PNP-Fuc-α(1-2)Gal-β(1-3)GlcNAc; Chromogenic substrate for α-L-fucosidase; 4-Nitrophenyl Fucα1-2Galβ1-3GlcNAcβ; Trisaccharide substrate 93496-53-8.
EINECS	Contact for details

Quality Control

Every batch of our biochemical substrates is produced and tested under stringent quality management protocols. We guarantee high purity and batch-to-batch consistency critical for research reproducibility and diagnostic accuracy. Comprehensive analysis, including HPLC for purity and identity confirmation, is performed. A detailed Certificate of Analysis (COA) specifying purity, assay results, and impurity profiles is provided with each shipment to ensure full traceability and compliance with your quality standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Assay (Enzymatic)	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.