Description

6-Carboxyfluorescein n-Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent widely used in biochemistry and molecular biology. Its core value lies in its ability to form stable amide bonds with primary amines, facilitating the sensitive detection and quantification of biomolecules. This compound is essential for researchers and manufacturers in the fields of bioconjugation, immunoassay development, nucleic acid labeling, and flow cytometry.

Application

• Protein and Antibody Labeling: Covalent conjugation to lysine residues for fluorescence-based detection in assays like ELISA and Western blotting.
• Oligonucleotide and Nucleic Acid Probes: Labeling of amine-modified DNA or RNA for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Cell Tracing and Tracking: Staining of cellular proteins for monitoring cell proliferation, migration, and viability in flow cytometry and microscopy.
• Bioconjugation and Linker Chemistry: Serving as a key intermediate for synthesizing fluorescently tagged peptides, polymers, and nanoparticles.
• Diagnostic Kit Manufacturing: Production of fluorescent reagents and tracers for clinical diagnostic and research kits.
• High-Throughput Screening (HTS): Development of fluorescent probes for enzymatic activity assays and receptor-ligand binding studies.

Basic Information

Product Name	6-Carboxyfluorescein n-Succinimidyl Ester
CAS No.	92557-81-8
Molecular Formula	C25H15NO9
Molecular Weight	473.39 g/mol
Synonyms	6-FAM, SE; 6-Carboxyfluorescein N-hydroxysuccinimide ester; 5(6)-Carboxyfluorescein N-succinimidyl ester; 6-CF SE; Fluorescein-6-isothiocyanate isomer; 6-FAM NHS ester; 6-Carboxyfluorescein succinimidyl ester; FAM, SE isomer 6
EINECS	Contact for details

Quality Control

Our 6-Carboxyfluorescein n-Succinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometry to ensure optimal fluorescence properties. Certificates of Analysis (COA) detailing purity, identity, and performance data are available upon request to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	492 - 498 nm
Emission Maximum	515 - 525 nm
Moisture Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.