Description

Mono-Methyl Polyethylene Glycol 5'000Acetic Acid n-Succinimidyl Ester is a high-purity, heterobifunctional PEGylation reagent designed for the site-specific conjugation of biomolecules. This compound is critical for creating stable, biologically active conjugates by linking the NHS ester group to primary amines while providing a methoxy-PEG chain to enhance solubility, reduce immunogenicity, and prolong circulation time. It is an essential tool for researchers and manufacturers in pharmaceutical development, bioconjugation, and advanced drug delivery systems, particularly for creating next-generation therapeutics like antibody-drug conjugates (ADCs) and PEGylated proteins.

Application

• Pharmaceutical PEGylation: Site-specific modification of therapeutic peptides, proteins, and enzymes to improve pharmacokinetic profiles.
• Antibody-Drug Conjugate (ADC) Development: As a linker component to attach cytotoxic payloads to monoclonal antibodies.
• Bioconjugation & Labeling: Covalent attachment of fluorescent dyes, tags, or other functional groups to biomolecules for diagnostics and imaging.
• Nanoparticle & Liposome Functionalization: Surface modification to enhance stealth properties and target specificity of drug delivery carriers.
• Surface Chemistry & Biomaterial Coating: Creating non-fouling, biocompatible surfaces for medical devices and implants.
• Crosslinking Agent: For the controlled crosslinking of amine-containing polymers or hydrogels.

Basic Information

Product Name	Mono-Methyl Polyethylene Glycol 5'000Acetic Acid n-Succinimidyl Ester
CAS No.	92451-01-9
Molecular Formula	C2n+5H4n+9NOn+4 (for mPEG5k-NHS)
Molecular Weight	~5000 g/mol (nominal)
Synonyms	mPEG5k-NHS Ester; mPEG5000-NHS; Methoxy PEG NHS Ester (MW 5000); α-Methyl-ω-(N-succinimidyl acetate) poly(ethylene glycol); PEG5000-Methyl Ether N-Hydroxysuccinimide Ester; NHS-PEG5k-OMe; N-Hydroxysuccinimide ester of methoxy poly(ethylene glycol) acetic acid; Succinimidyl ester of methoxy PEG propionic acid (MW 5000)
EINECS	Contact for details

Quality Control

Our Mono-Methyl Polyethylene Glycol 5'000Acetic Acid n-Succinimidyl Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent molecular weight, and functional group integrity. Certificates of Analysis (COA) are provided, detailing specifications for purity (by HPLC/NMR), degree of substitution, and residual solvents. We support development under GMP-grade conditions upon request for critical pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) to minimize hydrolysis of the NHS ester group. This material is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store under inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white waxy solid or powder
Identification (IR)	Conforms to structure
Molecular Weight (GPC)	5000 ± 10% (Mn)
Purity (HPLC)	≥ 95%
Degree of Substitution (NMR)	≥ 90%
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines
Functional Activity	Conforms (by amine coupling test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.