Description

Exotoxin A CAS NO 91262-95-2 is a potent protein toxin produced by the bacterium *Pseudomonas aeruginosa*, known for its specific enzymatic activity as an ADP-ribosyltransferase. This compound is of significant value in biomedical research for studying bacterial pathogenesis, host-pathogen interactions, and the mechanisms of toxin action. It serves as a critical reagent for researchers and developers in the fields of microbiology, immunology, and pharmacology, particularly for vaccine development, therapeutic antibody production, and cytotoxicity studies.

Application

• Biomedical Research: Fundamental studies on the mechanism of action, cellular uptake, and intracellular trafficking of bacterial toxins.
• Vaccine Development: Used as an antigen or a toxoid in the research and production of vaccines targeting *Pseudomonas aeruginosa* infections.
• Therapeutic Antibody Production: Serves as a target antigen for generating and screening monoclonal or polyclonal antibodies for therapeutic applications.
• Cytotoxicity Assays: Employed in in vitro models to study its effects on eukaryotic cells, including induction of apoptosis and inhibition of protein synthesis.
• Diagnostic Reagent Development: Used in the development of immunoassays and diagnostic kits for detecting *P. aeruginosa* infection or exposure.
• Enzymology Studies: Investigation of ADP-ribosylation reactions and their role in disrupting normal cellular function.

Basic Information

Product Name	Exotoxin A
CAS No.	91262-95-2
Molecular Formula	CnHnNnOnSn (Protein)
Molecular Weight	~66,000 Da (Monomer)
Synonyms	Pseudomonas Exotoxin A; PE; PE24; PE38; PE40; ETA; ADP-ribosylating exotoxin; Exotoxin A (Pseudomonas aeruginosa); ToxA
EINECS	Contact for details

Quality Control

Our Exotoxin A is produced and tested under stringent conditions to ensure high purity and biological activity suitable for research applications. Each batch is characterized by multiple analytical techniques, including SDS-PAGE, HPLC, and functional activity assays. A comprehensive Certificate of Analysis (COA) detailing purity, endotoxin levels, and specific activity is provided with every shipment to guarantee traceability and compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C to -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed under anhydrous conditions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band corresponding to ~66 kDa
Purity (HPLC)	≥ 95%
Protein Content (BCA)	≥ 90%
Endotoxin Level (LAL)	< 1.0 EU/µg
Specific Activity (Cell-based assay)	Contact for details
Solubility	Soluble in water or PBS
Moisture Content (KF)	< 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.