Description

Thrombin Metz CAS NO 90453-22-8 is a highly purified, lyophilized preparation of the serine protease thrombin, derived from bovine plasma. This critical enzyme plays a fundamental role in the final stage of the blood coagulation cascade, converting soluble fibrinogen into insoluble fibrin strands to form a stable clot. It is an essential biochemical reagent for research, diagnostic, and specialized bioprocessing applications. Key industries that rely on this product include life science research, in vitro diagnostics (IVD) manufacturing, and the development of hemostatic agents and biomaterials.

Application

• Life Science Research: Used as a key reagent in coagulation studies, enzyme kinetics, and biochemical pathway analysis.
• In Vitro Diagnostic (IVD) Manufacturing: A critical component in the production of test kits for coagulation panels, such as prothrombin time (PT) and activated partial thromboplastin time (aPTT) assays.
• Hemostatic Agent Formulation: Incorporated into topical hemostats, surgical sealants, and wound dressings to control bleeding during surgical procedures.
• Biomaterial & Tissue Engineering: Employed to cross-link fibrin-based scaffolds, hydrogels, and other biomaterials for cell culture and regenerative medicine applications.
• Cell Culture & Biotechnology: Used to detach adherent cells from culture surfaces in a gentle, enzymatic manner as an alternative to trypsin.
• Protein Biochemistry: Serves as a model protease for studying enzyme specificity, inhibition, and protein-protein interactions.

Basic Information

Product Name	Thrombin Metz
CAS No.	90453-22-8
Molecular Formula	Contact for details
Molecular Weight	~36,000 Da (Monomer)
Synonyms	Bovine Thrombin; Fibrinogenase; Coagulation Factor IIa; Activated Blood-Coagulation Factor II; Thrombin, Bovine; Thrombofort; Topical Thrombin; EINECS 291-588-6
EINECS	291-588-6

Quality Control

Our Thrombin Metz is manufactured under strict quality systems to ensure batch-to-batch consistency and high biological activity. Each lot is tested to meet rigorous specifications for purity, potency, and sterility where applicable. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing key parameters such as specific activity, clotting time, and purity by SDS-PAGE. We adhere to relevant quality guidelines suitable for research and in vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store lyophilized powder at 2-8°C. Upon reconstitution, aliquot and store solutions at -20°C or below to maintain activity. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (IR)	Conforms to Standard
Specific Activity	≥ 1000 NIH units/mg protein
Purity (SDS-PAGE)	≥ 90%
Clotting Time	Meets release criteria
pH (Reconstituted Solution)	6.5 - 7.5
Moisture Content (KF)	≤ 5.0 %
Bacterial Endotoxins	< 0.1 EU/ NIH unit

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.