Description

2,3-Di-(4-Nitrophenyl)-5-Phenyl-2H-Tetrazolium is a specialized tetrazolium salt widely recognized for its role as a vital biochemical reagent. This compound matters for its ability to act as a sensitive chromogenic substrate in enzymatic assays, producing a measurable formazan dye upon reduction. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies for applications in cell viability testing, microbiology, and dehydrogenase enzyme activity studies.

Application

• Cell Viability and Proliferation Assays (e.g., MTT assay alternatives): Used as a substrate to quantify metabolically active cells.
• Microbiological Testing: Employed in diagnostic kits to detect bacterial contamination or specific enzymatic activities in microbial cultures.
• Dehydrogenase Enzyme Activity Studies: Serves as an electron acceptor in biochemical research to measure the activity of various dehydrogenase enzymes.
• Histochemistry and Cytochemistry: Utilized as a staining agent to localize enzyme activity in tissue sections and cell cultures.
• Antioxidant Capacity Assays: Applied in research to evaluate the radical scavenging activity of compounds.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) platforms to identify potential drug candidates affecting cellular metabolism.

Basic Information

Product Name	2,3-Di-(4-Nitrophenyl)-5-Phenyl-2H-Tetrazolium
CAS No.	90376-02-6
Molecular Formula	C25H17N7O4
Molecular Weight	479.45 g/mol
Synonyms	2,3-Bis(4-nitrophenyl)-5-phenyl-2H-tetrazolium; 2,3-Di(4-nitrophenyl)-5-phenyltetrazolium; 2,3-Di-p-nitrophenyl-5-phenyltetrazolium; 2,3-Di(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride (common salt form); TNPT; p-Nitrotetrazolium Blue (related compound); Tetrazolium salt, nitrophenyl-substituted; INT (Iodonitrotetrazolium) analog
EINECS	Contact for details

Quality Control

Our 2,3-Di-(4-Nitrophenyl)-5-Phenyl-2H-Tetrazolium is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via FT-IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent absorption of moisture. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.