Description

5-Fluorouracil-6-D1 is a deuterium-labeled analog of the widely used chemotherapeutic agent 5-fluorouracil, specifically designed for advanced research and analytical applications. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, enabling precise pharmacokinetic and metabolic studies. It is a critical tool for pharmaceutical researchers, analytical chemists, and contract research organizations (CROs) engaged in drug development, bioanalytical method validation, and metabolism research.

Application

• Internal Standard for LC-MS/MS: Serves as a critical internal standard for the accurate quantification of 5-fluorouracil in complex biological matrices like plasma, serum, and tissue homogenates.
• Drug Metabolism and Pharmacokinetics (DMPK): Used in tracer studies to investigate the absorption, distribution, metabolism, and excretion (ADME) profile of 5-fluorouracil without isotopic interference.
• Bioanalytical Method Development: Essential for developing and validating robust, high-sensitivity HPLC-MS/MS methods in compliance with GLP and FDA guidelines.
• Clinical Research: Supports therapeutic drug monitoring (TDM) and clinical trial sample analysis to ensure accurate patient data.
• Isotope Dilution Mass Spectrometry (IDMS): Enables highly precise and accurate quantification in analytical chemistry, a gold-standard technique.
• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for quality control and assay calibration in pharmaceutical manufacturing.

Basic Information

Product Name	5-Fluorouracil-6-D1
CAS No.	90344-84-6
Molecular Formula	C4H2D1FN2O2
Molecular Weight	131.08 g/mol
Synonyms	5-Fluoro-6-deuterio-2,4(1H,3H)-pyrimidinedione; 5-FU-6-D1; [6-2H]-5-Fluorouracil; 5-Fluorouracil (6-D); Deuterated 5-Fluorouracil; 5-FU-d1; 2,4-Dihydroxy-5-fluoro-6-deuteriopyrimidine; 5-Fluoro-1H,3H-pyrimidine-2,4-dione-6-d
EINECS	Contact for details

Quality Control

Our 5-Fluorouracil-6-D1 is manufactured under strict quality control protocols to ensure the highest isotopic and chemical purity for critical research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing results for isotopic purity (D-enrichment), chemical purity (by HPLC), and identification (by NMR and MS). We support research compliance with standards relevant to reference materials and can provide custom QC data packages upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.