Description

Superose 6B is a high-performance, cross-linked agarose gel filtration chromatography medium designed for the high-resolution separation of biomolecules. Its primary value lies in providing excellent resolution for large proteins, protein complexes, and other macromolecules in the 5,000 to 5,000,000 molecular weight range. This product is essential for research and development laboratories, as well as process development teams in the biotechnology and pharmaceutical industries, requiring reliable and reproducible size-exclusion chromatography.

Application

• Size-exclusion chromatography (SEC) or gel filtration chromatography of proteins, antibodies, and other biomolecules.
• Purification and analysis of monoclonal antibodies and antibody fragments.
• Separation of protein complexes, viruses, and other large macromolecular assemblies.
• Desalting and buffer exchange of protein samples in preparative workflows.
• Molecular weight estimation and analysis of biopolymers in research settings.
• Process development and scale-up for downstream biopharmaceutical purification.
• Fractionation of polysaccharides, nucleic acids, and synthetic polymers.

Basic Information

Product Name	Superose 6B
CAS No.	90093-39-3
Molecular Formula	(C12H18O9)n
Molecular Weight	High molecular weight polymer
Synonyms	Agarose, cross-linked; Sepharose 6B; Gel filtration medium 6B; Cross-linked beaded agarose; 6% Cross-linked agarose; Bio-Gel A-5m (similar type); Size exclusion chromatography resin; Macroporous agarose gel
EINECS	Contact for details

Quality Control

Our Superose 6B is manufactured under strict quality systems to ensure batch-to-batch consistency critical for chromatographic performance. Each lot is tested for key parameters including particle size distribution, flow rate, exclusion limits, and chemical stability. Certificates of Analysis (COA) detailing these specifications are provided to guarantee the material meets the high standards required for analytical and preparative bioseparations.

Storage

Preserve in a tightly closed container, protected from light. Store the suspension in 20% ethanol at 4°C to 8°C (39°F to 46°F) to prevent microbial growth and maintain chromatographic performance. Do not freeze. For long-term storage of dried media, keep in a cool, dry place at room temperature (15°C to 25°C).

Specification

Item	Specification
Matrix	Cross-linked 6% beaded agarose
Particle Size	45-165 μm (wet)
Exclusion Limit (Globular Proteins)	~5 x 10⁶ Da
Separation Range (Globular Proteins)	5,000 - 5,000,000 Da
Maximum Operating Pressure	0.3 MPa (3 bar, 43 psi)
pH Stability (long term)	3 - 12
pH Stability (short term)	2 - 14
Chemical Stability	Stable in most aqueous buffers, detergents, and 6M guanidine HCl. Avoid strong oxidizing agents.
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.