Description

Hemoglobin M is a stable, oxidized form of hemoglobin where the iron in the heme group is in the ferric (Fe³⁺) state, rendering it incapable of binding oxygen. This compound, identified by CAS No. 9083-28-7, serves as a critical biochemical standard and research reagent for studying hemoglobinopathies and oxidative stress in biological systems. It is essential for researchers and manufacturers in the fields of clinical diagnostics, hematology research, and the development of calibration materials for medical devices.

Application

• Biochemical Research: Used as a standard and model compound for investigating methemoglobinemia, enzyme kinetics (e.g., methemoglobin reductase), and oxidative damage to red blood cells.
• Clinical Diagnostics: Serves as a reference material in the calibration and validation of laboratory instruments that measure methemoglobin levels in blood.
• Pharmaceutical Development: Employed in studies related to drug-induced oxidative stress and the development of therapeutic agents targeting hemoglobin disorders.
• Educational & Reference Standards: Provides a high-purity standard for academic teaching and quality control in the production of diagnostic kits.
• Toxicology Studies: Utilized in research on the effects of oxidizing chemicals and toxins on hemoglobin function.

Basic Information

Product Name	Hemoglobin M
CAS No.	9083-28-7
Molecular Formula	C2952H4664N812O832S8Fe4 (Approximate for α2β2 tetramer)
Molecular Weight	~64,500 Da (for tetramer)
Synonyms	Methemoglobin; Ferrihemoglobin; Hemiglobin; Oxidized Hemoglobin; Hi; HbM; Hemoglobin (ferric); Myohemoglobin (ferric); Erythrocruorin (ferric)
EINECS	Contact for details

Quality Control

Our Hemoglobin M is produced and handled under strict quality protocols to ensure batch-to-batch consistency and high purity for research applications. Each lot is characterized to confirm its identity and oxidation state. A comprehensive Certificate of Analysis (COA) detailing purity, spectral properties, and functional characteristics is provided with every shipment to support your quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. This product is easily oxidized; handle under an inert atmosphere when possible for critical applications.

Specification

Item	Specification
Appearance	Brownish-red powder or solution
Identification (UV-Vis)	Spectrum consistent with methemoglobin standard
Purity (HPLC)	≥ 95% (Methemoglobin form)
Protein Content (Biuret)	≥ 90% of total weight
Ferric Iron Content	≥ 95% of total heme iron
Solubility	Soluble in aqueous buffers, pH ~7.0
Endotoxin	< 1.0 EU/mg (if required)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.