Description

Succinate-Coa Ligase (Adp-Forming) is a critical enzyme that catalyzes the reversible conversion of succinate and coenzyme A to succinyl-CoA, a key intermediate in the citric acid cycle. This enzymatic activity is fundamental to cellular energy metabolism and biosynthetic pathways. It is essential for researchers and manufacturers in the fields of biochemical research, diagnostic reagent production, and pharmaceutical development, where precise metabolic pathway analysis and high-purity enzyme preparations are required.

Application

• Biochemical Research & Assay Development: Used as a critical reagent in enzymatic assays to study the citric acid cycle (Krebs cycle), mitochondrial function, and cellular energy metabolism.
• Diagnostic Kit Manufacturing: Incorporated into in-vitro diagnostic (IVD) kits for the clinical measurement of metabolites related to metabolic disorders, such as succinate or CoA derivatives.
• Pharmaceutical R&D: Employed in drug discovery and development pipelines targeting metabolic diseases, cancer metabolism, and mitochondrial dysfunction.
• Enzyme Engineering & Biocatalysis: Serves as a model enzyme or a starting point for protein engineering efforts aimed at creating novel biocatalysts for industrial chemical synthesis.
• Academic & Educational Use: Supplied to universities and teaching laboratories for practical courses in biochemistry, enzymology, and molecular biology.
• Metabolomics & Systems Biology: Utilized in complex reaction mixtures to validate metabolic network models and flux analysis studies.

Basic Information

Product Name	Succinate-Coa Ligase (Adp-Forming)
CAS No.	9080-33-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Succinyl-CoA synthetase (ADP-forming); Succinate-CoA ligase (ADP-forming); SCS-ADP; EC 6.2.1.5; Succinyl coenzyme A synthetase; Succinyl-CoA ligase; Succinate thiokinase (ADP); GTP-specific succinyl-CoA synthetase (misnomer)
EINECS	Contact for details

Quality Control

Our enzyme preparations undergo rigorous quality testing to ensure high specific activity, purity, and batch-to-batch consistency. Each lot is characterized for activity (U/mg protein) and screened for contaminating enzymatic activities. Certificates of Analysis (COA) detailing specific activity, purity (via SDS-PAGE), protein concentration, and buffer composition are available upon request. Production follows quality management principles suitable for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture uptake, which can affect activity and stability.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Identification (Activity)	Conforms
pH (in solution)	7.0 - 7.5
Stabilizers / Buffer	Potassium phosphate, DTT, Glycerol (typical)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.