Description

Cytochrome B5, Human CAS NO 9078-32-4 is a highly purified, recombinant heme protein essential for electron transfer in biological systems. This product is critical for research and development in biochemistry and pharmacology, offering a reliable standard for studying metabolic pathways and enzyme interactions. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in drug metabolism studies, protein engineering, and diagnostic assay development.

Application

• Biochemical Research: Serves as a critical electron donor in studies of cytochrome P450 enzymes and other redox systems.
• Drug Metabolism Studies: Used in in vitro assays to investigate phase I metabolism and drug-drug interactions.
• Diagnostic Reagent Manufacturing: A key component in the production of reagents for clinical diagnostics and biosensors.
• Enzyme Reconstitution: Essential for reactivating and studying the function of fatty acid desaturases and other membrane-bound enzymes.
• Reference Standard: Provides a high-purity standard for quality control in pharmaceutical and biotech production.
• Academic & Educational Use: Employed in university laboratories for teaching advanced concepts in enzymology and bioenergetics.

Basic Information

Product Name	Cytochrome B5, Human
CAS No.	9078-32-4
Molecular Formula	C42H58FeN8O6S2 (Heme core approximate)
Molecular Weight	~15.8 kDa (Protein moiety)
Synonyms	Cytochrome b5; CYB5A; Microsomal Cytochrome b5; Heme Protein b5; Recombinant Human Cytochrome B5; Methemoglobin Reductase; Ferrocytochrome b5; Apocytochrome b5; B5 Cytochrome
EINECS	Contact for details

Quality Control

Our Cytochrome B5, Human is produced under strict quality management systems. Each batch is subjected to rigorous analytical testing including HPLC for purity, UV-Vis spectroscopy for heme content and redox state verification, and SDS-PAGE for molecular weight confirmation. Certificates of Analysis (COA) detailing purity, activity, and endotoxin levels are available upon request to ensure compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For short-term use, store at 2-8°C.

Specification

Item	Specification
Appearance	Dark red to brown lyophilized powder
Identification (UV-Vis)	Spectrum characteristic of Cytochrome B5
Purity (HPLC)	≥ 95%
Protein Content (Biuret)	≥ 90%
Heme Content (Pyridine Hemochrome)	≥ 0.8 mol heme/mol protein
Specific Activity	Contact for details
Solubility	Soluble in aqueous buffers
Endotoxin	< 1.0 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.