Description

Carboxypeptidase G is a highly specific zinc-containing exopeptidase enzyme that catalyzes the hydrolysis of C-terminal glutamate residues from folic acid analogs. This precise catalytic activity makes it a critical reagent in biochemical research and pharmaceutical development, particularly for targeted drug metabolism studies. It is essential for laboratories and production facilities in the biotechnology, pharmaceutical, and life science sectors requiring enzymatic cleavage of glutamyl linkages.

Application

• Biochemical Research: Used as a tool enzyme for studying peptide and protein structure, function, and metabolism.
• Pharmaceutical Development: Employed in the metabolism and clearance studies of antifolate drugs and other glutamate-conjugated compounds.
• Enzyme Therapy Research: Investigated for potential therapeutic applications in targeted drug activation systems.
• Diagnostic Reagent Manufacturing: A component in the production of specialized diagnostic kits and assay systems.
• Biocatalysis: Utilized in synthetic chemistry for the selective removal of glutamic acid protecting groups.
• Quality Control Testing: Applied in analytical laboratories for the characterization of folate-based pharmaceuticals and biomolecules.

Basic Information

Product Name	Carboxypeptidase G
CAS No.	9074-87-7
Molecular Formula	N/A (Protein/Enzyme)
Molecular Weight	Approx. 41,000 - 46,000 Da (Monomer)
Synonyms	Carboxypeptidase G; CPG; Glutamate Carboxypeptidase; Folate Hydrolase; Pteroylpoly-γ-glutamate Carboxypeptidase; γ-Glutamyl Hydrolase; EC 3.4.17.11; Conjugase
EINECS	Contact for details

Quality Control

Our Carboxypeptidase G is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is characterized for specific activity, purity, and absence of contaminating proteases. A comprehensive Certificate of Analysis (COA) detailing assay results, physical characteristics, and stability data is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to prevent activity loss. Due to its hygroscopic nature, the lyophilized material should be equilibrated to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms
Specific Activity	≥ 50 units/mg protein
Protein Content (Biuret)	≥ 90%
Purity (SDS-PAGE)	Single major band
Solubility	Clear, colorless solution in buffer
pH (1% solution)	6.0 - 7.5
Loss on Drying	≤ 5.0%
Bacterial Endotoxins	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.