Description

Sucrose Phosphorylase is a highly specific glycosyltransferase enzyme that catalyzes the reversible phosphorolysis of sucrose to yield D-fructose and α-D-glucose-1-phosphate. This biocatalyst is essential for the efficient, stereospecific synthesis of α-glucosides and other valuable carbohydrate derivatives under mild conditions. It is a critical tool for researchers and manufacturers in the biotechnology, pharmaceutical, and food ingredient sectors, enabling the production of specialty sugars, prebiotics, and pharmaceutical intermediates with high purity and yield.

Application

• Prebiotic Synthesis: Enzymatic production of functional oligosaccharides like kojibiose and other rare sugars.
• Pharmaceutical Intermediates: Synthesis of glycosylated compounds and prodrugs for enhanced bioavailability.
• Biocatalysis & Process Development: Used in enzymatic cascade reactions and the development of green chemistry manufacturing processes.
• Food & Beverage Ingredient Production: Manufacturing of novel sweeteners, flavor modifiers, and texturizing agents.
• Diagnostic Reagents: Component in enzymatic assay kits for sucrose or phosphate detection.
• Biochemical Research: Study of carbohydrate metabolism, enzyme kinetics, and glycosylation mechanisms.

Basic Information

Product Name	Sucrose Phosphorylase
CAS No.	9074-06-0
Molecular Formula	N/A (Enzyme)
Molecular Weight	~50-55 kDa (Source dependent)
Synonyms	Sucrose: Orthophosphate α-D-Glucosyltransferase; Sucrose Phosphorylase (E.C. 2.4.1.7); Sucrose Glucosyltransferase; Sucrose-Phosphate Glucosyltransferase; α-Glucose-1-Phosphate:D-Fructose 2-α-D-Glucosyltransferase; Leuconostoc mesenteroides Sucrose Phosphorylase; SPase
EINECS	Contact for details

Quality Control

Our Sucrose Phosphorylase is produced and tested under stringent quality management systems. Each batch is characterized for critical parameters including specific activity, purity, and stability to ensure reproducible performance in your applications. We provide comprehensive Certificates of Analysis (COA) detailing assay results, and our quality systems support compliance with relevant standards for research and industrial use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> 20 U/mg protein (Typical)
Protein Content (Biuret)	> 90%
Purity (SDS-PAGE)	> 95%
pH Optimum	6.0 - 7.0
Temperature Optimum	30 - 40 °C
Stabilizers	May contain buffer salts

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.