Description

Thermolysin is a thermostable metalloprotease enzyme derived from the bacterium *Bacillus thermoproteolyticus*. This enzyme is critically valued for its high specificity in cleaving peptide bonds on the amino side of hydrophobic amino acids, making it an indispensable tool in biochemical research and industrial bioprocessing. It is essential for professionals in the pharmaceutical, food technology, and life science research sectors who require precise protein digestion, peptide synthesis, or flavor enhancement processes.

Application

• Peptide Synthesis & Protein Sequencing: Used for the specific fragmentation of proteins to generate peptides for mass spectrometry analysis and Edman degradation.
• Pharmaceutical Intermediate Production: Employed in the enzymatic synthesis of aspartame and other peptide-based pharmaceutical intermediates.
• Food Industry Processing: Applied in cheese manufacturing for flavor development and in protein hydrolysate production to improve functional properties.
• Biochemical Research: A key reagent for studying protein structure-function relationships, enzyme kinetics, and protease mechanisms.
• Cell Culture & Tissue Dissociation: Utilized for the gentle dissociation of specific tissues in primary cell culture preparations.
• Diagnostic Kit Manufacturing: Incorporated as a component in enzymatic assay kits and diagnostic reagents.

Basic Information

Product Name	Thermolysin
CAS No.	9073-78-3
Molecular Formula	N/A (Protein)
Molecular Weight	Approx. 34,600 Da
Synonyms	Thermolysin; TLN; Bacillus thermoproteolyticus neutral protease; Thermoase; Thermolysin from *Bacillus thermoproteolyticus* rokko; Thermolysin, Microbial; Neutral Metalloproteinase; EC 3.4.24.27
EINECS	Contact for details

Quality Control

Our Thermolysin is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as proteolytic activity, purity profile, and absence of specified impurities. We support compliance with relevant standards for research and industrial use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive). For long-term stability, keep desiccated. Handle under controlled conditions to prevent moisture absorption and activity loss.

Specification

Item	Specification
Appearance	Lyophilized powder or crystalline suspension
Identification (IR)	Conforms
Activity	> 50,000 U/mg protein
Purity (HPLC)	> 95%
Loss on Drying	< 5.0 %
Heavy Metals	< 20 ppm
Microbial Limits	Meets specification for non-sterile enzyme

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.