Description

Glucosidase, Exo-1,3-β- is a highly specific hydrolytic enzyme that catalyzes the cleavage of terminal 1,3-β-D-glucosidic linkages. This precise catalytic activity is critical for the controlled degradation and analysis of complex β-glucan polysaccharides found in various biological and industrial matrices. It is an essential research and process tool for biochemists, molecular biologists, and industrial scientists working in carbohydrate chemistry, biofuels research, and the development of functional food ingredients.

Application

• Biochemical Research & Enzyme Characterization: Used as a model enzyme for studying the kinetics and mechanisms of β-glucan hydrolysis.
• Structural Analysis of Polysaccharides: Essential tool for the sequential degradation and structural elucidation of complex β-glucans like laminarin, pachyman, and other fungal/cereal-derived polysaccharides.
• Biofuel Production: Employed in enzymatic cocktails for the saccharification of lignocellulosic biomass, specifically targeting hemicellulose components containing β-1,3-glucans.
• Food & Nutraceutical Industry: Used to modify β-glucan structures to alter solubility, viscosity, or to produce specific oligosaccharides with prebiotic potential.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assay kits designed to quantify β-glucan levels, particularly for applications in clinical mycology (fungal infection detection) and food quality control.
• Plant & Fungal Physiology Studies: Applied in research to understand the role of β-glucan metabolism in cell wall remodeling, defense responses, and developmental processes.

Basic Information

Product Name	Glucosidase, Exo-1,3-β-
CAS No.	9073-49-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details (Enzyme)
Synonyms	Exo-1,3-β-Glucosidase; 1,3-β-D-Glucan Exo-hydrolase; Exo-β-1,3-Glucanase; Laminarinase (Exo-type); Exo-1,3-β-D-Glucanase; β-1,3-Glucan Exo-hydrolase; EC 3.2.1.58
EINECS	Contact for details

Quality Control

Our enzyme preparations undergo rigorous quality testing to ensure high specific activity, purity, and batch-to-batch consistency. Quality is verified through a suite of analytical methods including activity assays, protein content determination (Bradford/Lowry), and SDS-PAGE for purity assessment. Certificates of Analysis (COA) detailing specific activity (U/mg), protein concentration, and buffer composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake. For optimal activity retention, prepare working aliquots to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	> X U/mg protein (Contact for specific grade)
Protein Content (Bradford)	> X% (w/w)
Purity (SDS-PAGE)	Single major band
Optimum pH	~5.0 (Substrate dependent)
Optimum Temperature	~40°C (Substrate dependent)
Stabilizers/Buffer	May contain salts or glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.