Description

Dispase CAS NO 9068-59-1 is a neutral, non-mammalian metalloprotease enzyme derived from *Bacillus polymyxa*, specifically engineered for the gentle dissociation of cells and tissues. Its primary value lies in its ability to cleave fibronectin and collagen IV at a neutral pH without damaging cell surface proteins, ensuring high cell viability post-dissociation. This product is essential for researchers and manufacturers in the biotechnology and pharmaceutical industries, particularly for applications in primary cell culture, stem cell research, and tissue engineering.

Application

• Primary Cell Isolation and Culture: Gentle dissociation of epithelial cells, hepatocytes, keratinocytes, and other sensitive cell types from various tissues.
• Stem Cell Research: Critical for the passaging and harvesting of embryonic stem (ES) and induced pluripotent stem (iPS) cells while maintaining pluripotency.
• Tissue Engineering & Regenerative Medicine: Used to dissociate tissues for the generation of cell sheets and 3D constructs.
• Organoid Culture: Facilitates the processing of tissue fragments for the establishment and maintenance of complex organoid models.
• Cancer Research: Isolation of tumor cells from biopsy specimens for primary culture and downstream analysis.
• Mammary Gland Biology: Specific use in the isolation of mammary epithelial cells for lactation and development studies.

Basic Information

Product Name	Dispase
CAS No.	9068-59-1
Molecular Formula	N/A (Enzyme Preparation)
Molecular Weight	~35-40 kDa (Protein component)
Synonyms	Dispase II; Neutral Protease Dispase; Bacillus polymyxa Neutral Protease; Microbial Neutral Metalloprotease; EC 3.4.24.4; Fibronectinase; Collagenase Type IV (specific activity); Tissue Dissociation Enzyme
EINECS	Contact for details

Quality Control

Our Dispase is manufactured under strict quality systems to ensure batch-to-batch consistency and performance for critical research and production applications. Each lot is tested for specific activity, purity, and the absence of contaminating proteases (e.g., trypsin, clostripain). A comprehensive Certificate of Analysis (COA) detailing assay results, sterility (where applicable), and endotoxin levels is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term stability, store desiccated. Avoid repeated freeze-thaw cycles of prepared solutions.

Specification

Item	Specification
Appearance	White to light brown lyophilized powder
Identification (IR)	Conforms
Specific Activity	≥ 4.0 units/mg protein (Casein substrate)
Protein Content (Biuret)	≥ 50%
pH (1% Solution)	6.0 - 7.5
Loss on Drying	≤ 5.0 %
Endotoxin (LAL)	< 0.1 EU/µg (for cell culture grade)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.