Description

3',5'-Cyclic-Gmp Phosphodiesterase is a crucial enzyme that catalyzes the hydrolysis of the second messenger cyclic GMP (cGMP) to 5'-GMP, playing a pivotal role in regulating intracellular signaling pathways. This enzyme is essential for research focused on understanding cellular communication, signal transduction mechanisms, and phosphodiesterase inhibitor development. It is primarily utilized by biochemical researchers and pharmaceutical developers in life science research, drug discovery, and diagnostic assay development.

Application

• Biochemical Research: Fundamental studies on cyclic nucleotide signaling, enzyme kinetics, and cellular regulation pathways.
• Pharmaceutical R&D: Target enzyme for screening and developing novel phosphodiesterase inhibitors as potential therapeutics.
• Diagnostic Kit Manufacturing: A key component in enzymatic assay kits for measuring cGMP levels in biological samples.
• Cell Signaling Studies: Investigation of nitric oxide (NO) and natriuretic peptide signaling cascades where cGMP is a secondary messenger.
• Enzyme Characterization: Substrate specificity studies and comparative analysis with other phosphodiesterase isoforms.
• Academic & Industrial Laboratories: Used as a standard or reagent in various experimental protocols requiring precise modulation of cGMP concentration.

Basic Information

Product Name	3',5'-Cyclic-Gmp Phosphodiesterase
CAS No.	9068-52-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	cGMP Phosphodiesterase; Cyclic GMP Phosphodiesterase; PDE (cGMP-specific); Guanosine 3',5'-cyclic monophosphate phosphodiesterase; 3',5'-cGMP PDE; cGMP-PDE; PDE5 (related activity); Type V Phosphodiesterase (related isoform); EC 3.1.4.35
EINECS	Contact for details

Quality Control

Our 3',5'-Cyclic-Gmp Phosphodiesterase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high specific activity, purity, and functional consistency. A comprehensive Certificate of Analysis (COA) is provided, detailing parameters such as specific activity, protein content, and purity assessed by SDS-PAGE. Our quality commitment aligns with standards suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Enzyme Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Protein Content (Bradford)	Reported per batch
Purity (SDS-PAGE)	≥ 90%
pH (for solutions)	7.0 - 7.5
Buffer Composition	Reported per batch
Stability	Stable for 12 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.