Description

Dehydrogenase, Malate (Pig Heart) is a highly purified enzyme preparation derived from porcine cardiac tissue, specifically catalyzing the reversible oxidation of malate to oxaloacetate. This biocatalyst is critical for research and diagnostic applications requiring precise NAD+/NADH-linked enzymatic activity. It is an essential reagent for biochemists, clinical researchers, and diagnostic manufacturers working in metabolic pathway analysis and enzyme-coupled assays. The product is supplied as a lyophilized powder to ensure stability and consistent performance.

Application

• Clinical Diagnostics: Key component in enzymatic assay kits for the quantitative determination of metabolites like malate, aspartate, or related enzymes in serum and plasma.
• Biochemical Research: Used in studies of the citric acid (Krebs) cycle, gluconeogenesis, and amino acid metabolism to investigate enzymatic mechanisms and kinetics.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify potential modulators of metabolic pathways for drug discovery.
• Food & Beverage Analysis: Applied in enzymatic methods for the accurate quantification of malic acid and other organic acids in quality control laboratories.
• Educational & Teaching Kits: Supplied as a reliable, active enzyme for university-level laboratory experiments demonstrating enzyme kinetics and metabolic biochemistry.
• Biotechnology: Utilized in coupled enzyme systems for the synthesis of fine chemicals or for biosensor development.

Basic Information

Product Name	Dehydrogenase, Malate (Pig Heart)
CAS No.	9067-93-0
Molecular Formula	Protein (Enzyme)
Molecular Weight	~70,000 Da (Subunit composition varies)
Synonyms	Malate Dehydrogenase (MDH); L-Malate:NAD+ Oxidoreductase; Heart Malate Dehydrogenase; Porcine Heart MDH; NAD+-dependent Malate Dehydrogenase; EC 1.1.1.37; Malic Dehydrogenase
EINECS	Contact for details

Quality Control

Our Dehydrogenase, Malate (Pig Heart) is manufactured under strict quality management systems. Each lot is tested to ensure high specific activity, purity, and functional stability. Certificates of Analysis (COA) detailing parameters such as specific activity (U/mg protein), protein content, and contaminant enzyme activities are provided with every shipment. We adhere to relevant guidelines for biological reagent production to ensure batch-to-batch consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the lyophilized powder at -20°C. Once reconstituted, aliquot and store the solution at -20°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (Activity)	Catalyzes the oxidation of L-Malate
Specific Activity	≥ 1,200 U/mg protein (at 25°C, pH 9.5)
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	Single major band
Contaminating Activities	Lactate Dehydrogenase (LDH): ≤ 0.01% Glutamate Dehydrogenase (GDH): ≤ 0.01%
Solubility	Completely soluble in water or buffer
Stability	≥ 2 years at -20°C (lyophilized)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.