Description

Phosphoenolpyruvate Carboxylase CAS NO 9067-77-0 is a critical enzyme that catalyzes the irreversible carboxylation of phosphoenolpyruvate (PEP) to oxaloacetate, a fundamental reaction in carbon fixation and anaplerotic pathways. This biocatalyst is essential for research and industrial processes requiring precise control of C4 metabolism and the replenishment of TCA cycle intermediates. It is primarily utilized by biochemists, molecular biologists, and process engineers in the biotechnology, pharmaceutical, and agricultural research sectors.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic assays to study metabolic pathways, enzyme kinetics, and carbon flux analysis.
• Diagnostic Kit Manufacturing: Incorporated into clinical diagnostic kits for the measurement of metabolites like pyruvate, ATP, or other enzymes in serum.
• Metabolic Engineering: Employed in synthetic biology and metabolic engineering projects to enhance carbon fixation and improve yields in microbial production of chemicals and biofuels.
• Pharmaceutical Intermediate Synthesis: Serves as a biocatalyst in the enzymatic synthesis of fine chemicals and chiral intermediates for active pharmaceutical ingredients (APIs).
• Agricultural Biotechnology: Applied in plant science research to engineer crops with improved photosynthetic efficiency and stress tolerance.
• Food & Beverage Analysis: Utilized in enzymatic methods for the quality control and analysis of specific components in food products.

Basic Information

Product Name	Phosphoenolpyruvate Carboxylase
CAS No.	9067-77-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PEP Carboxylase; PEPCase; Phosphoenolpyruvate Carboxylase (PEPC); Phosphopyruvate Carboxylase; PEP Carboxykinase (in some contexts); EC 4.1.1.31; Orthophosphate:oxaloacetate carboxy-lyase (phosphorylating)
EINECS	Contact for details

Quality Control

Our Phosphoenolpyruvate Carboxylase is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity, and stability to ensure reliable performance in sensitive applications. A comprehensive Certificate of Analysis (COA), detailing parameters such as activity units/mg protein, protein concentration, and buffer composition, is provided to guarantee traceability and batch-to-batch consistency for our B2B partners.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect enzymatic activity. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers / Buffer	Typically in Tris or phosphate buffer with stabilizers
Activity Assay Method	Spectrophotometric (NADH oxidation)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.