Description

Sephadex, 2-(Diethylamino)Ethyl Ether is a functionalized, cross-linked dextran gel widely used as an anion exchange chromatography medium for the separation and purification of biomolecules. Its key value lies in its high capacity for binding negatively charged proteins, nucleic acids, and other anions under mild, non-denaturing conditions. This product is essential for researchers and process developers in the biotechnology, pharmaceutical, and life science industries requiring high-resolution purification.

Application

• Protein Purification: Primary application for the isolation and purification of enzymes, antibodies, and other proteins from complex biological mixtures.
• Nucleic Acid Separation: Used for the fractionation of RNA, DNA, and oligonucleotides based on charge differences.
• Biopharmaceutical Downstream Processing: A critical tool in the purification train for therapeutic proteins, vaccines, and other biologics at laboratory, pilot, and production scales.
• Enzyme Immobilization: Serves as a solid support matrix for the covalent attachment of enzymes for use in biocatalysis and industrial processes.
• Desalting and Buffer Exchange: Can be utilized for the rapid removal of salts and small molecules from protein solutions.
• Academic and Industrial Research: Fundamental tool in biochemistry and molecular biology labs for analytical and preparative separations.

Basic Information

Product Name	Sephadex, 2-(Diethylamino)Ethyl Ether
CAS No.	9064-92-0
Molecular Formula	(C6H10O5)n•[C2H4N(C2H5)2]
Molecular Weight	Variable (Polymeric Bead)
Synonyms	DEAE-Sephadex; Sephadex DEAE; Diethylaminoethyl-Sephadex; 2-(Diethylamino)ethyl ether of cross-linked dextran; DEAE-Dextran gel; Anion exchanger Sephadex; Dextran, 2-(diethylamino)ethyl ether, cross-linked; DEAE-Sephadex A-25; DEAE-Sephadex A-50
EINECS	Contact for details

Quality Control

Our Sephadex DEAE is manufactured under a strict quality management system. Each lot is tested to ensure consistent performance characteristics, including ion exchange capacity, particle size distribution, and flow properties. While specific grades may vary, our products are suitable for research and process development use. Certificates of Analysis (COA) detailing lot-specific parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect performance. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white free-flowing beads
Ion Exchange Capacity	3.5 ± 0.5 meq/g (dry weight) - Typical
Particle Size (wet)	40 - 120 μm (Grade dependent)
Exclusion Limit (Globular Proteins)	~30,000 Da (Grade dependent)
Swelling Factor (in water)	5 - 10 ml/g (dry gel)
pH Stability (operational)	2 - 9 (long term), 2 - 11 (short term)
Identification (IR)	Conforms to structure

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.