Description

Cytochrome B558 CAS NO 9064-78-2 is a critical membrane-bound component of the NADPH oxidase complex, essential for generating reactive oxygen species (ROS) in phagocytic cells. This high-purity protein is vital for research into innate immunity, oxidative stress, and chronic granulomatous disease (CGD). It is primarily utilized by biopharmaceutical R&D teams, academic researchers, and diagnostic manufacturers focused on immunology, biochemistry, and respiratory burst studies.

Application

• Biochemical Research: Essential reagent for studying the structure, function, and assembly of the NADPH oxidase complex in vitro.
• Immunology & Inflammation Studies: Used to investigate the molecular mechanisms of phagocyte-mediated host defense and inflammatory signaling pathways.
• Diagnostic Development: Serves as a key component in the development of assays for diagnosing Chronic Granulomatous Disease (CGD) and other phagocyte disorders.
• Drug Discovery & Screening: Employed as a target protein in high-throughput screening (HTS) platforms to identify potential modulators of NADPH oxidase activity for therapeutic applications.
• Enzyme Kinetics & Mechanism Studies: Provides a purified source for detailed kinetic analysis of electron transfer processes within the oxidase complex.
• Antioxidant Research: Used in studies evaluating the effects of antioxidants and inhibitors on superoxide anion production.

Basic Information

Product Name	Cytochrome B558
CAS No.	9064-78-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cytochrome b-558; Phagocyte Cytochrome b; NADPH Oxidase Cytochrome b Subunit; gp91phox/p22phox Heterodimer; Superoxide-Generating NADPH Oxidase Light Chain; Flavocytochrome b558; Heme Protein b-558; Neutrophil Cytochrome b
EINECS	Contact for details

Quality Control

Our Cytochrome B558 is produced and handled under stringent quality protocols to ensure batch-to-batch consistency and biological activity. Each lot is characterized for purity, heme content, and functional activity in a reconstituted NADPH oxidase system. A comprehensive Certificate of Analysis (COA) detailing specifications such as spectral purity (A428/A280 ratio), SDS-PAGE analysis, and functional assay results is provided with every shipment to support your research compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive) and easily oxidized; therefore, it should be handled under an inert atmosphere when possible and reconstituted with degassed buffers.

Specification

Item	Specification
Appearance	Dark red to brown solution or lyophilized powder
Identification (Spectral Scan)	Characteristic Soret peak at ~428 nm; α/β bands at ~558/530 nm
Purity (SDS-PAGE)	≥ 90% (under reducing conditions)
Spectral Purity (A428/A280)	≥ 0.8
Protein Concentration	As per COA (Bradford/BCA assay)
Functional Activity	Superoxide generation in a cell-free assay system (nmol/min/mg protein)
Solvent/Buffer	Specified on COA (e.g., Tris buffer with glycerol)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.