Description

Histone Type Viii-S is a highly purified, lyophilized preparation of histone proteins derived from calf thymus, widely recognized for its role in chromatin structure and epigenetic research. This product is essential for scientists studying nucleosome assembly, histone modifications, and gene regulation mechanisms. It serves as a critical reagent for biochemical assays, enzyme substrate studies, and in vitro chromatin reconstitution experiments in academic, pharmaceutical, and biotechnology R&D sectors.

Application

• Chromatin Research & Nucleosome Assembly: Used as a core component for in vitro reconstitution of nucleosomes to study chromatin dynamics and structure.
• Enzyme Substrate for Modifying Enzymes: Serves as a substrate for histone acetyltransferases (HATs), deacetylases (HDACs), methyltransferases, kinases, and ubiquitin ligases in activity assays.
• Biochemical & Biophysical Studies: Employed in protein-protein interaction studies, gel shift assays (EMSA), and analytical techniques like chromatography and electrophoresis.
• Epigenetics & Drug Discovery: A key tool in high-throughput screening for identifying and characterizing epigenetic modulators and therapeutic agents.
• Antibody Production & Validation: Used as an antigen for generating and testing specificity of antibodies against various histone isoforms and modifications.
• Cell Biology & Functional Genomics: Applied in studies investigating the role of histones in transcription, DNA repair, and replication.

Basic Information

Product Name	Histone Type Viii-S
CAS No.	9064-47-5
Molecular Formula	Mixture of proteins
Molecular Weight	~11-16 kDa (core histone monomers)
Synonyms	Calf Thymus Histone Type VIII-S; Histone Fraction VIII-S; Histone Proteins (Calf Thymus); Total Histones; Core Histones (H2A, H2B, H3, H4); Histone Preparation Type VIII-S; Nucleosomal Histones
EINECS	Contact for details

Quality Control

Our Histone Type Viii-S is manufactured under controlled conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity assessment by electrophoresis (SDS-PAGE) and functional activity validation in standard biochemical assays. Certificates of Analysis (COA) detailing purity, protein concentration, and biological activity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated at -20°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Electrophoresis)	Consistent banding pattern for core histones (H2A, H2B, H3, H4)
Purity (SDS-PAGE)	≥ 90% (Total core histones)
Protein Content (Biuret)	≥ 95% of dry weight
Solubility	Soluble in water or dilute buffer (e.g., 10 mM Tris-HCl, pH 7.5)
Biological Activity	Suitable for nucleosome assembly and as a substrate for histone-modifying enzymes
Endotoxin Level	< 1.0 EU/mg (for research-grade applications)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.