Description

Nucleoside Phosphorylase Bacterial is a purified enzyme preparation derived from bacterial sources, specifically catalyzing the reversible phosphorolysis of nucleosides. This biocatalyst is essential for the enzymatic synthesis of nucleoside analogs, a critical class of compounds in modern pharmaceutical development. It is primarily utilized by research institutions and manufacturers in the biotechnology and pharmaceutical industries for the production of antiviral and anticancer agents.

Application

• Enzymatic Synthesis of Nucleoside Analogs: Key biocatalyst for the scalable, stereoselective production of modified nucleosides used in antiviral (e.g., for HIV, Hepatitis) and anticancer therapies.
• Biocatalytic Process Development: Employed in green chemistry and process chemistry routes to develop more efficient and sustainable manufacturing pathways for active pharmaceutical ingredients (APIs).
• Biochemical Research & Assay Development: Used as a critical reagent in metabolic pathway studies, enzyme kinetics research, and the development of diagnostic assays.
• Precursor Production for mRNA Therapeutics: Facilitates the synthesis of specialized nucleoside precursors required for manufacturing mRNA vaccines and therapeutics.
• Library Generation for Drug Discovery: Enables the generation of diverse nucleoside libraries for high-throughput screening in early-stage drug discovery programs.

Basic Information

Product Name	Nucleoside Phosphorylase Bacterial
CAS No.	9059-37-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Purine Nucleoside Phosphorylase (Bacterial); PNPase; Inosine Phosphorylase; Nucleoside: Orthophosphate Ribosyltransferase; EC 2.4.2.1; Bacterial Nucleoside Phosphorylase; DeoD-type Purine Nucleoside Phosphorylase
EINECS	Contact for details

Quality Control

Our Nucleoside Phosphorylase Bacterial is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high catalytic activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing parameters such as specific activity, protein content, and purity. We support compliance with cGMP guidelines for advanced pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under controlled humidity conditions to maintain stability and activity. For long-term storage, consider keeping the product at -20°C.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ XX U/mg protein
Protein Content (Biuret)	≥ XX %
Purity (SDS-PAGE)	≥ XX %
pH (in solution)	6.0 - 8.0
Endotoxin (LAL)	< 1.0 EU/mg
Bioprocess Contaminants	Negative for host cell proteins & DNA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.