Description

p-Hydroxybenzoate Hydroxylase is a specialized enzyme that catalyzes the hydroxylation of p-hydroxybenzoate, a key step in the microbial degradation pathway of aromatic compounds. This biocatalyst is essential for research and industrial processes focused on bioremediation, metabolic engineering, and the synthesis of valuable phenolic intermediates. It is primarily utilized by biotechnology firms, academic research institutions, and companies developing enzymatic solutions for environmental and pharmaceutical applications.

Application

• Bioremediation Research: Study and development of microbial pathways for the degradation of aromatic pollutants in environmental samples.
• Metabolic Engineering: A key enzymatic tool for constructing or modifying microbial biosynthetic pathways for fine chemicals.
• Enzymology Studies: Used in biochemical research to investigate enzyme kinetics, mechanism, and structure-function relationships.
• Biosensor Development: Potential component in enzymatic assays or sensors for detecting specific aromatic compounds.
• Pharmaceutical Intermediate Synthesis: Enabling biocatalytic steps in the production of complex drug molecules or their precursors.
• Academic & Industrial R&D: Fundamental research tool in microbiology, biochemistry, and green chemistry laboratories.

Basic Information

Product Name	p-Hydroxybenzoate Hydroxylase
CAS No.	9059-23-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	4-Hydroxybenzoate 3-Monooxygenase; 4-Hydroxybenzoate 3-Hydroxylase; p-Hydroxybenzoate 3-Hydroxylase; 4-Hydroxybenzoate Hydroxylase; PHBH; EC 1.14.13.2; 4-Hydroxybenzoic Acid 3-Hydroxylase
EINECS	Contact for details

Quality Control

Our p-Hydroxybenzoate Hydroxylase is produced and tested under stringent quality management protocols to ensure high activity, purity, and batch-to-batch consistency. Each lot is characterized for specific activity and protein content. A comprehensive Certificate of Analysis (COA), detailing purity, activity units, and storage buffer specifications, is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use to prevent moisture absorption.

Specification

Item	Specification
Appearance	Lyophilized powder or clear solution
Identification (Activity)	Catalyzes hydroxylation of p-hydroxybenzoate
Specific Activity	≥ [Value] units/mg protein
Protein Content (Bradford)	≥ [Value] %
Purity (SDS-PAGE)	≥ [Value] %
Storage Buffer	Specified phosphate or Tris buffer, pH ~7.5
Stabilizers	May contain glycerol or other stabilizers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.