Description

Cystathionine β-Lyase (Cbl) is a pivotal pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the final step in the transsulfuration pathway, converting cystathionine to cysteine, α-ketobutyrate, and ammonia. This enzymatic activity is essential for cellular redox balance, hydrogen sulfide (H₂S) production, and methionine metabolism, making it a critical reagent for biochemical research and diagnostic applications. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical development, and clinical diagnostics sectors.

Application

• Biochemical Research: A key tool for studying the transsulfuration pathway, sulfur amino acid metabolism, and the biological roles of hydrogen sulfide (H₂S).
• Pharmaceutical Development: Used in target validation and screening assays for drug discovery programs focused on cardiovascular diseases, neurological disorders, and cancer where H₂S signaling is implicated.
• Diagnostic Kit Manufacturing: Employed as a core component in enzymatic assay kits for the quantitative determination of L-cystathionine or related metabolites in clinical and research samples.
• Enzyme Kinetics Studies: Serves as a model enzyme for investigating PLP-dependent reaction mechanisms and inhibitor interactions.
• Metabolic Pathway Analysis: Used in in vitro systems to reconstruct and analyze metabolic fluxes through the transsulfuration pathway.
• Nutritional Science Research: Applied in studies investigating sulfur amino acid requirements and metabolism in various biological models.

Basic Information

Product Name	Cystathionine β-Lyase (Cbl)
CAS No.	9055-55-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cystathionase; L-Cystathionine β-lyase; Cystathionine γ-lyase (common alias); CSE; Cystathionine γ-lyase (often used interchangeably); EC 4.4.1.8; L-Cystathionine cysteine-lyase (deaminating); Homoserine dehydratase (obsolete)
EINECS	Contact for details

Quality Control

Our Cystathionine β-Lyase is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. Specifications typically include assessment by SDS-PAGE, activity assays, and protein content determination. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	≥ [Value] %
Purity (SDS-PAGE)	≥ [Value] %
pH (in solution)	7.0 - 8.0
Endotoxin (LAL)	< [Value] EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.