Description

Prekallikrein CAS NO 9055-02-1 is a pivotal serine protease zymogen, also known as Fletcher Factor, that plays a critical role in the initiation of the intrinsic blood coagulation pathway and the kallikrein-kinin system. Its primary commercial value lies in its essential function as a high-purity biochemical reagent for advanced diagnostic and research applications. This product is indispensable for manufacturers and research institutions in the in-vitro diagnostics (IVD), pharmaceutical research, and life science sectors, particularly for developing coagulation assays, studying inflammatory pathways, and producing reference standards.

Application

• In-Vitro Diagnostic (IVD) Reagents: A key component in the manufacturing of test kits and calibrators for assessing the intrinsic coagulation pathway, such as activated partial thromboplastin time (APTT) tests.
• Biochemical Research: Used as a critical substrate or reagent in enzymatic studies to investigate the contact activation system, bradykinin generation, and related inflammatory processes.
• Pharmaceutical Development: Employed in drug discovery and screening programs targeting coagulation disorders, hereditary angioedema, and sepsis.
• Reference Standard Production: Serves as a purified standard for quality control in diagnostic manufacturing and for calibrating laboratory equipment.
• Enzyme Substrate: Acts as a specific precursor for the generation of plasma kallikrein in controlled experimental settings.

Basic Information

Product Name	Prekallikrein
CAS No.	9055-02-1
Molecular Formula	Contact for details
Molecular Weight	~85,000 - 88,000 Da (Single-chain glycoprotein)
Synonyms	Fletcher Factor; Plasma Prekallikrein; PK; PKK; Kallikreinogen; Coagulation Factor Fletcher; Pre-Kallikrein; Contact Activation Coagulation Factor; EC 3.4.21.-
EINECS	Contact for details

Quality Control

Our Prekallikrein is manufactured and tested under a rigorous quality management system to ensure batch-to-batch consistency and high biological activity for research and diagnostic use. Each lot is characterized for purity, functional activity, and absence of significant contaminants. A comprehensive Certificate of Analysis (COA) detailing specific test results, including assay by chromogenic substrate, SDS-PAGE purity, and sterility/endotoxin levels where applicable, is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (Functional Activity)	Converts to Kallikrein upon activation with appropriate activator (e.g., FXIIa, dextran sulfate)
Purity (SDS-PAGE)	≥ 90% (Single chain, under reducing conditions)
Specific Activity (Chromogenic Assay)	≥ [Value] U/mg protein
Protein Content	Determined by A280 or other suitable method
Solubility	Reconstitutes clearly in appropriate buffer (e.g., Tris, PBS)
Endotoxin (if sterile filtered)	< 1.0 EU/μg (or per customer requirement)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.