Description

Sephadex, 2-Hydroxypropyl Ether is a hydrophilic, cross-linked dextran derivative widely used as a size-exclusion chromatography medium. This product, with CAS number 9049-22-3, is valued for its excellent chemical stability and predictable fractionation range, enabling precise biomolecule separation. It is an essential tool for researchers and process engineers in the biotechnology, pharmaceutical, and life science industries for purifying proteins, peptides, nucleic acids, and polysaccharides.

Application

• Size-Exclusion Chromatography (SEC/GFC): Primary application for the separation and purification of biomolecules like proteins, enzymes, antibodies, and peptides based on their hydrodynamic volume.
• Desalting and Buffer Exchange: Efficient removal of salts, dyes, or other small molecules from protein and nucleic acid samples in laboratory and process-scale workflows.
• Pharmaceutical Downstream Processing: Critical step in the purification of therapeutic proteins, vaccines, and other biologics to meet stringent purity requirements.
• Molecular Weight Determination: Used to estimate the molecular weight of unknown proteins and polymers by comparison with standard calibration curves.
• Sample Preparation for Analysis: Prepares clean samples for downstream analytical techniques such as HPLC, mass spectrometry, and electrophoresis.
• Oligonucleotide and Plasmid DNA Purification: Separation of nucleic acid fragments and removal of contaminants in molecular biology applications.
• Industrial Enzyme Purification: Isolation and purification of enzymes used in food processing, biofuel production, and other industrial biocatalysis.

Basic Information

Product Name	Sephadex, 2-Hydroxypropyl Ether
CAS No.	9049-22-3
Molecular Formula	(C6H10O5)n • (C3H6O)x
Molecular Weight	Variable (Polymer)
Synonyms	2-Hydroxypropyl Ether of Cross-linked Dextran; Sephadex LH-20; Hydroxypropyl Sephadex; LH-20; Dextran, 2-hydroxypropyl ether, cross-linked; LH Sephadex; Lipophilic Sephadex; G-25, Hydroxypropyl Derivative
EINECS	Contact for details

Quality Control

Our Sephadex, 2-Hydroxypropyl Ether is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency and performance. Key parameters such as particle size distribution, exclusion limits, swelling volume, and functional group substitution are strictly controlled. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect swelling properties and performance.

Specification

Item	Specification
Appearance	White to off-white free-flowing powder
Identification (IR)	Conforms to standard
Particle Size (wet)	Specified grade (e.g., 20-80 μm, 40-120 μm)
Exclusion Limit (Mw)	Grade-specific (e.g., ~4,000 for peptides)
Swelling Volume	4-6 mL/g (in water or specified solvent)
Hydroxypropyl Substitution	Contact for details
pH (in water)	5.0 - 7.5
Heavy Metals	< 10 ppm
Loss on Drying	< 7.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.