Description

Carboxymethyl Sephadex CAS NO 9047-08-9 is a functionalized derivative of the cross-linked dextran gel, Sephadex, where carboxymethyl groups are introduced to impart weak cation exchange properties. This modification is critical for enabling the selective separation and purification of biomolecules, particularly proteins and peptides, based on differences in their net charge. It is an essential tool for researchers and process engineers in the biotechnology, pharmaceutical development, and life sciences sectors who require high-resolution chromatographic media for downstream processing and analytical applications.

Application

• Ion-Exchange Chromatography (IEC): Primary use as a weak cation exchanger for the purification of positively charged proteins, enzymes, and polypeptides.
• Buffer Exchange and Desalting: Efficient removal of salts and small molecules from protein solutions while changing buffer conditions.
• Laboratory-Scale Protein Purification: A key media for research and development workflows in academic and industrial labs for isolating target biomolecules.
• Pilot and Process-Scale Biomanufacturing: Used in the capture and intermediate purification steps during the production of therapeutic proteins and vaccines.
• Enzyme Immobilization: Serves as a functional matrix for covalently binding enzymes via its carboxyl groups for use in biocatalysis.
• Sample Preparation for Analysis: Pre-cleaning and fractionation of complex biological samples prior to techniques like HPLC or mass spectrometry.

Basic Information

Product Name	Carboxymethyl Sephadex
CAS No.	9047-08-9
Molecular Formula	(C6H10O5)n with -CH2COOH substituents
Molecular Weight	Polymeric substance (Variable)
Synonyms	CM-Sephadex; Carboxymethyl Sephadex Ion Exchanger; Sephadex CM; Carboxymethylated Cross-linked Dextran; CM-Dextran Gel; Weak Cation Exchanger Sephadex; Dextran, 2-(carboxymethyl) ether, cross-linked
EINECS	Contact for details

Quality Control

Our Carboxymethyl Sephadex is manufactured under a quality management system to ensure batch-to-batch consistency for critical chromatographic performance. Each lot is tested for key parameters including ion exchange capacity, particle size distribution, swelling factor, and functional integrity. Certificates of Analysis (COA) detailing these specifications are provided with every shipment. While not typically compendial, production can be aligned with principles of GMP for pharmaceutical applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed immediately after use to prevent moisture uptake, which can affect swelling properties and performance. Avoid freezing.

Specification

Item	Specification
Appearance	White to off-white free-flowing powder
Ion Exchange Capacity	3.5 - 5.0 meq/g (dry weight)
Particle Size (wet)	40 - 120 μm (Fractionated grades available)
Swelling Factor (in water)	5 - 10 ml/g
Functional Group	Carboxymethyl (Weak cation exchanger)
pH Stability (working range)	2 - 10
Maximum Operating Pressure	0.3 MPa
Identification (IR)	Conforms to structure

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.