Description

Sephadex G-25 is a cross-linked dextran gel filtration medium designed for group separation and desalting of biomolecules. This product is essential for the rapid and efficient removal of low molecular weight contaminants, such as salts, dyes, and nucleotides, from proteins, peptides, and nucleic acids. It is a critical tool for researchers and process engineers in the biotechnology, pharmaceutical, and diagnostic industries requiring high-purity sample preparation.

Application

• Desalting and Buffer Exchange of proteins, antibodies, and oligonucleotides in downstream bioprocessing.
• Group Separation for removing unincorporated nucleotides or dyes from labeled DNA probes.
• Sample Clean-up prior to analytical techniques like HPLC, mass spectrometry, or electrophoresis.
• Fractionation of biomolecules in the molecular weight range of 1,000 to 5,000 Da.
• Removal of phenol, chaotropic salts, and other small molecules from nucleic acid preparations.
• Used in the preparation of enzyme substrates and purification of synthetic peptides.
• Support for diagnostic kit manufacturing where consistent separation performance is required.
• Laboratory-scale and pilot-scale purification processes in GMP-compliant environments.

Basic Information

Product Name	Sephadex G-25
CAS No.	9041-35-4
Molecular Formula	(C6H10O5)n
Molecular Weight	High polymer (varies by bead size and cross-linking)
Synonyms	Dextran gel; Cross-linked dextran; Gel filtration medium G-25; Sephadex G25; Dextran bead; Gel chromatography medium; Size exclusion chromatography medium; Bio-Gel P equivalent (for comparison)
EINECS	Contact for details

Quality Control

Our Sephadex G-25 is manufactured under strict quality systems to ensure batch-to-batch consistency for critical separations. Each lot is tested for key performance parameters including particle size distribution, exclusion limit, swelling volume, and flow characteristics. Certificates of Analysis (COA) are provided, detailing specifications relevant to pharmaceutical and research applications. We support compliance with cGMP, ISO 9001, and REACH requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent moisture uptake, which can affect swelling properties and performance.

Specification

Item	Specification
Appearance	White to off-white free-flowing beads
Particle Size (dry)	20-80 μm (Fine) / 50-150 μm (Medium) / 100-300 μm (Coarse)
Exclusion Limit (globular proteins)	~5,000 Da
Bed Volume (swollen in water)	4-6 mL/g dry gel
pH Stability (long-term)	2 - 13
Operating Temperature	4°C - 110°C (wet)
Conductivity	Low ionic content
Microbial Control	Low bioburden

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.