Description

Exonuclease Iii CAS NO 9037-44-9 is a processive 3'→5' exonuclease enzyme that specifically digests double-stranded DNA from blunt or recessed 3'-termini. This product is essential for molecular biology applications requiring the controlled removal of nucleotides or the generation of single-stranded templates. It is a critical reagent for researchers and developers in biotechnology, pharmaceutical R&D, and academic laboratories working on DNA sequencing, mutagenesis, and genetic analysis.

Application

• DNA Sequencing Sample Preparation: Used to generate single-stranded DNA templates for traditional Sanger sequencing by digesting one strand.
• Site-Directed Mutagenesis: Employed to create gapped DNA duplexes, facilitating the incorporation of mutagenic oligonucleotides.
• DNA-Protein Interaction Studies: Assists in footprinting assays by digesting DNA not bound by protective proteins.
• Removal of Contaminating Nucleic Acids: Useful in the purification of other enzymes or biological samples where residual DNA must be eliminated.
• Molecular Cloning: Aids in the construction of nested deletions for mapping functional domains or sequencing large DNA inserts.
• Next-Generation Sequencing (NGS) Library Construction: Can be utilized in specific protocols for end-repair or size selection steps.

Basic Information

Product Name	Exonuclease III
CAS No.	9037-44-9
Molecular Formula	N/A (Enzyme)
Molecular Weight	~28,000 Da (Monomer)
Synonyms	Exodeoxyribonuclease III; Exo III; EC 3.1.11.2; Deoxyribonuclease (pyrimidine dimer); Escherichia coli Exonuclease III; DNA deoxyribophosphodiesterase; AP endonuclease; 3'→5' exonuclease
EINECS	Contact for details

Quality Control

Our Exonuclease III is produced under stringent conditions to ensure high purity and consistent enzymatic activity. Each lot undergoes rigorous quality testing, including assays for specific activity, nuclease contamination, and functional performance in standard applications. A Certificate of Analysis (COA) detailing purity, activity units, and storage buffer composition is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C in a non-frost-free freezer. The enzyme is supplied in a glycerol-containing buffer to prevent freezing. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is sealed tightly after each use.

Specification

Item	Specification
Appearance	Clear, colorless solution
Concentration	10,000 units/mL (Typical)
Specific Activity	>100,000 units/mg protein
Purity (SDS-PAGE)	>95%
Endonuclease Activity	Not detectable
Ribonuclease Activity	Not detectable
Phosphatase Activity	Not detectable
Storage Buffer	10 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.