Description

Cytochrome O is a vital heme-containing protein complex, a member of the cytochrome family, which plays a crucial role in the electron transport chain of certain bacterial respiratory systems. Its significance lies in its function as a terminal oxidase, facilitating the final step of aerobic respiration and contributing to cellular energy production. This makes it an essential biochemical reagent for researchers in microbiology, enzymology, and bioenergetics, particularly for studying bacterial metabolism, oxidative phosphorylation, and as a model system for membrane protein research.

Application

• Biochemical Research: Used as a key reagent in studies of bacterial electron transport chains and terminal oxidases.
• Enzymology Studies: Serves as a model protein for investigating enzyme kinetics, heme-cofactor interactions, and redox mechanisms.
• Microbial Metabolism Analysis: Employed in research focused on aerobic respiration pathways in specific bacterial species.
• Membrane Protein Research: Acts as a standard or subject in studies concerning the structure and function of integral membrane proteins.
• Educational & Diagnostic Kits: May be utilized as a component in advanced educational laboratory kits or specialized diagnostic assays for research purposes.
• Biofuel & Biocatalyst Development: Investigated for potential applications in bioelectrochemical systems and the development of novel biocatalysts.

Basic Information

Product Name	Cytochrome O
CAS No.	9035-48-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cytochrome o; Cytochrome bo; Ubiquinol oxidase; Terminal oxidase bo; Cytochrome bo3; Heme-copper oxidase bo; Cytochrome bd-I (related complex); Bacterial cytochrome o; Proton-pumping quinol oxidase
EINECS	Contact for details

Quality Control

Our Cytochrome O is produced and handled under stringent quality management protocols to ensure batch-to-batch consistency and reliability for critical research applications. Each lot is characterized for purity and activity using advanced analytical techniques. A comprehensive Certificate of Analysis (COA) detailing specific parameters such as spectral purity, specific activity, and protein concentration is provided with every shipment to support your research documentation and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product should be handled under appropriate laboratory conditions to maintain its biological activity.

Specification

Item	Specification
Appearance	Dark brown to red-brown solution or lyophilized powder
Identification (UV-Vis)	Complies (Characteristic Soret and alpha/beta bands)
Purity (SDS-PAGE)	> 90% (Single major band)
Specific Activity	Contact for details (µmol O2 reduced/min/mg protein)
Protein Concentration	As per COA (Bradford or similar method)
Buffer Composition	Typically in Tris or Phosphate buffer with stabilizers
Endotoxin Level	Available upon request (for cell culture-grade material)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.