Description

Pyrophosphatase, Acid is a hydrolytic enzyme that specifically catalyzes the cleavage of inorganic pyrophosphate (PPi) into two orthophosphate (Pi) molecules. This reaction is critical for driving biosynthetic pathways forward by removing an inhibitory byproduct, thereby ensuring thermodynamic efficiency. It is an essential reagent for researchers and manufacturers in molecular biology, diagnostic assay development, and nucleotide synthesis, where precise control of phosphate metabolism is required.

Application

• Molecular Biology & Biochemistry Research: Used in enzymatic assays to study ATPases, polymerases, and other pyrophosphate-generating systems by eliminating PPi, which can inhibit reactions.
• Diagnostic Kits & Reagents: Incorporated into in vitro diagnostic (IVD) kits, such as those for nucleic acid amplification (PCR, sequencing), to prevent pyrophosphate-mediated inhibition and improve assay sensitivity and accuracy.
• Nucleotide & Oligonucleotide Synthesis: Employed in the production of high-purity nucleotides by cleaving pyrophosphate bonds, facilitating purification and increasing yield.
• Enzymatic Synthesis & Biocatalysis: Acts as a driving force in synthetic enzymatic pathways, making thermodynamically unfavorable reactions proceed efficiently.
• Food & Beverage Analysis: Utilized in enzymatic methods for the determination of inorganic pyrophosphate in food products.
• Pharmaceutical Development: Applied in drug discovery research targeting phosphate-metabolizing enzymes and in the synthesis of nucleotide-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	Pyrophosphatase, Acid
CAS No.	9033-44-7
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and preparation
Synonyms	Inorganic Pyrophosphatase; Acid Pyrophosphatase; Pyrophosphate Phospho-Hydrolase; PPase; EC 3.6.1.1; Inorganic Diphosphatase; Diphosphate Phosphohydrolase; Yeast Pyrophosphatase (common source)
EINECS	Contact for details

Quality Control

Our Pyrophosphatase, Acid is produced and tested under a rigorous quality management system. Each lot is characterized for specific activity, purity, and the absence of contaminating nucleases and phosphatases. Certificates of Analysis (COA) detailing assay results, stability data, and storage conditions are provided to ensure compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to minimize moisture condensation. Aliquot reconstituted enzyme to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Specific Activity	≥ XXX units/mg protein
Purity (SDS-PAGE)	≥ 95%
Protein Concentration	Defined per batch
Contaminants	Free of detectable RNase, DNase, and non-specific phosphatase activity
Buffer Composition	Defined per grade (e.g., Tris-HCl, Glycerol)
pH	7.0 - 7.5 (for solution)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.