Description

α-Ketoglutarate Dehydrogenase is a critical enzyme complex that catalyzes the oxidative decarboxylation of α-ketoglutarate to succinyl-CoA within the tricarboxylic acid (TCA) cycle. This reaction is fundamental to cellular energy metabolism, making the enzyme essential for research into mitochondrial function, metabolic disorders, and bioenergetics. It is a key reagent for biochemical research, diagnostic assay development, and enzyme activity studies in the life sciences and pharmaceutical industries.

Application

• Biochemical Research & Assay Development: Essential for studying the TCA cycle, mitochondrial respiration, and cellular metabolism in academic and industrial research laboratories.
• Diagnostic Kit Manufacturing: Used as a core component in enzymatic assay kits for clinical diagnostics, particularly for measuring related metabolites or enzyme activities in biological samples.
• Pharmaceutical R&D: Employed in drug discovery programs targeting metabolic pathways, cancer metabolism, and neurodegenerative diseases where mitochondrial dysfunction is implicated.
• Enzyme Activity & Inhibition Studies: Serves as a standard for quantifying enzyme activity and screening for potential inhibitors or activators in high-throughput screening (HTS) platforms.
• Metabolomics & Systems Biology: A critical tool for in vitro reconstitution of metabolic pathways to understand flux and regulation.
• Food & Agricultural Biotechnology: Potential applications in biosensor development for food quality control or in research on plant and microbial metabolism.

Basic Information

Product Name	α-Ketoglutarate Dehydrogenase
CAS No.	9031-02-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	2-Oxoglutarate Dehydrogenase; α-Ketoglutarate Dehydrogenase Complex; OGDC; KGDH; 2-Oxoglutarate Dehydrogenase (Lipoamide); α-Ketoglutarate Dehydrogenase (NAD+); EC 1.2.4.2; α-KGDH
EINECS	Contact for details

Quality Control

Our α-Ketoglutarate Dehydrogenase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. A comprehensive Certificate of Analysis (COA) detailing activity (U/mg), protein content, and purity assessments is provided with every shipment to guarantee reproducible performance for your critical research and manufacturing applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture uptake. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	Defined per batch
Purity (SDS-PAGE)	Major band corresponding to E1, E2, E3 subunits
Stabilizers / Buffer	Defined per grade
Activity Loss (at -20°C)	< 10% per year

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.