Description

D-Hydantoinase is a specialized biocatalyst enzyme critical for the stereoselective hydrolysis of 5-substituted hydantoins. This enzymatic activity is fundamental for the efficient, green synthesis of enantiomerically pure D-amino acids, which are valuable chiral building blocks. Industries such as pharmaceuticals, agrochemicals, and fine chemicals rely on this enzyme to produce high-purity intermediates for APIs, chiral herbicides, and advanced materials.

Application

• Pharmaceutical Intermediates: Key catalyst in the synthesis of D-p-hydroxyphenylglycine and D-phenylglycine, crucial side chains for semisynthetic antibiotics like ampicillin and amoxicillin.
• Chiral Synthesis: Production of non-natural D-amino acids used in peptide-based drugs and diagnostic agents.
• Agrochemical Manufacturing: Synthesis of enantiomerically pure active ingredients for herbicides and plant growth regulators.
• Fine Chemical Production: Enantioselective resolution of racemic hydantoins to obtain high-value specialty chemicals.
• Biocatalytic Process Development: Used in immobilized enzyme systems or whole-cell biocatalysts for continuous flow manufacturing.
• Research & Development: Tool enzyme in biochemical research for studying enzyme kinetics and metabolic pathways involving hydantoins.

Basic Information

Product Name	D-Hydantoinase
CAS No.	9030-74-4
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies (Protein)
Synonyms	D-Hydantoin Hydrolase; Hydantoin Racemase (in some systems); 5-Substituted-Hydantoinase; D-Specific Hydantoinase; Dihydropyrimidinase (EC 3.5.2.2); D-Hyd; DHTase; D-Hydantoin Hydrolyzing Enzyme
EINECS	Contact for details

Quality Control

Our D-Hydantoinase is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, protein content, and stereoselectivity to ensure consistent performance in biocatalytic processes. A comprehensive Certificate of Analysis (COA) detailing activity units (U/mg), purity, and stability data is provided with every shipment. Production can adhere to relevant ISO and cGMP guidelines for applications requiring regulatory filing.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized (freeze-dried) powder at -20°C. For short-term storage or in-use solutions, maintain at 2-8°C. This product is hygroscopic (moisture-sensitive); always allow the vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Catalyzes hydrolysis of D,L-5-phenylhydantoin
Specific Activity	≥ 15 U/mg protein (Contact for specific grade)
Protein Content (Biuret)	≥ 90%
Enantiomeric Excess (ee)	> 99% for D-product
pH Optimum	8.0 - 9.0
Temperature Optimum	40 - 50 °C
Stability (Lyophilized)	> 24 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.