Description

Nicotinamide Phosphoribosyltransferase is a crucial enzyme involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+), a vital coenzyme in cellular metabolism and energy production. Its primary function in catalyzing the rate-limiting step of the NAD+ salvage pathway makes it a significant target for research in metabolic regulation, aging, and various disease states. This high-purity enzyme is essential for biochemical research, pharmaceutical development, and diagnostic applications, serving scientists in academia, biotechnology, and the life sciences industry who require reliable and active protein reagents.

Application

• Biochemical Research: Used as a key reagent in enzymatic assays to study NAD+ metabolism, sirtuin activity, and cellular energy pathways.
• Drug Discovery & Screening: Serves as a target protein for high-throughput screening (HTS) to identify potential inhibitors or activators for metabolic disorders, cancer, and inflammatory diseases.
• Diagnostic Kit Development: Incorporated into in-vitro diagnostic (IVD) kits for the quantitative measurement of NAD+ levels or related metabolic markers in biological samples.
• Enzyme Kinetics Studies: Employed to determine kinetic parameters (Km, Vmax) and elucidate the catalytic mechanism of the NAD+ salvage pathway.
• Cell Biology & Aging Research: Utilized in cellular models to investigate the role of NAD+ biosynthesis in aging, mitochondrial function, and stress response.
• Protein-Protein Interaction Studies: Acts as a bait or prey protein in studies exploring its interactome and regulatory networks within the cell.

Basic Information

Product Name	Nicotinamide Phosphoribosyltransferase
CAS No.	9030-27-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	NAmPRTase; NAMPT; Pre-B-cell colony-enhancing factor 1 (PBEF1); Visfatin; Nicotinamide mononucleotide adenylyltransferase; EC 2.4.2.12; Nicotinate phosphoribosyltransferase (carboxylating)
EINECS	Contact for details

Quality Control

Our Nicotinamide Phosphoribosyltransferase is produced under stringent conditions to ensure high specific activity and purity, suitable for sensitive research applications. Each lot is subjected to rigorous quality testing, including activity assays, purity analysis, and stability checks. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, buffer composition, and storage conditions is provided with every shipment to guarantee traceability and compliance with your research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Lyophilized powder or clear solution
Purity (SDS-PAGE)	≥ 90%
Specific Activity	≥ X units/mg protein (Contact for details)
Protein Concentration	Determined by lot (See COA)
Buffer Composition	Tris-HCl, Glycerol, DTT (or similar stabilizers)
Endotoxin Level	< 1.0 EU/µg (for cell culture grades)
Identification (Activity Assay)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.