Description

Nucleoside Phosphorylase is a crucial enzyme that catalyzes the reversible phosphorolysis of nucleosides to their corresponding bases and pentose-1-phosphates. This biocatalyst is essential for nucleotide salvage pathways, enabling the efficient recycling of nucleosides and supporting critical cellular functions. It is a vital reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries, particularly for the synthesis of nucleoside analogs and biochemical research.

Application

• Pharmaceutical Synthesis: Key biocatalyst in the enzymatic production of nucleoside analogs used as antiviral and anticancer agents.
• Biocatalysis & Process Development: Employed in green chemistry routes for the stereoselective synthesis of modified nucleosides and nucleotides.
• Biochemical Research: Used in metabolic pathway studies, enzyme kinetics assays, and investigations into purine and pyrimidine metabolism.
• Diagnostic Reagent Manufacturing: A component in enzymatic assay kits for clinical diagnostics and research to measure nucleoside levels.
• Enzyme Engineering: Serves as a model enzyme for protein engineering studies aimed at improving stability, substrate specificity, or activity.
• Academic & Industrial R&D: Fundamental tool for studying enzyme mechanisms and developing new bioconversion processes.

Basic Information

Product Name	Nucleoside Phosphorylase
CAS No.	9030-21-1
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Purine Nucleoside Phosphorylase (PNP); Inosine Phosphorylase; Guanosine Phosphorylase; Nucleoside: Orthophosphate Ribosyltransferase; EC 2.4.2.1; PNPase; NPase; Nucleoside Phosphotransferase
EINECS	Contact for details

Quality Control

Our Nucleoside Phosphorylase is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and consistency. We provide detailed Certificates of Analysis (COA) that include data on activity (U/mg), protein content, and purity profiles. Our quality commitment supports applications requiring reliable enzymatic performance in GMP-aligned and research settings.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	> 10 U/mg protein (Typical)
Protein Content (Biuret)	Reported on COA
Purity (SDS-PAGE)	> 90%
pH (in solution)	7.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg (if required)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.