Description

Acetyl-CoA: Orthophosphate Acetyltransferase is a critical enzyme that catalyzes the reversible transfer of an acetyl group from acetyl-CoA to orthophosphate, forming acetyl phosphate and coenzyme A. This reaction is fundamental to cellular energy metabolism and biosynthetic pathways, making the enzyme an essential research tool for studying metabolic flux and enzyme kinetics. It is primarily utilized by biochemists, pharmaceutical researchers, and industrial biotechnologists working in metabolic engineering, diagnostic assay development, and high-throughput screening for drug discovery.

Application

• Biochemical Research & Enzyme Kinetics: Essential for in vitro studies of phosphate acetyltransferase activity, substrate specificity, and inhibitor screening.
• Metabolic Pathway Analysis: Used to investigate and reconstruct central metabolic pathways, particularly those involving acetyl-CoA and energy transfer.
• Diagnostic Kit Development: Serves as a key component in enzymatic assays for clinical diagnostics and research, measuring substrates or products linked to this enzymatic reaction.
• Pharmaceutical Screening: Employed in target-based assays to identify potential therapeutic compounds that modulate this enzyme's activity.
• Industrial Biotechnology: Applied in synthetic biology and metabolic engineering projects to optimize pathways for the production of biofuels or biochemicals.
• Academic & Educational Use: A standard reagent for teaching advanced concepts in biochemistry and enzymology at the university level.

Basic Information

Product Name	Acetyl-CoA: Orthophosphate Acetyltransferase
CAS No.	9029-91-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Phosphate Acetyltransferase; Acetyl-CoA:Pi Acetyltransferase; Phosphotransacetylase; PTA; Acetyl-CoA Acetyltransferase; EC 2.3.1.8; Acetyl Coenzyme A:Orthophosphate Acetyltransferase
EINECS	Contact for details

Quality Control

Our Acetyl-CoA: Orthophosphate Acetyltransferase is produced and tested under stringent quality management protocols. Each lot is characterized for specific activity, purity, and stability to ensure reproducible and reliable performance in sensitive applications. We provide comprehensive Certificates of Analysis (COA) detailing assay results, including activity units, protein content, and purity assessments. Our quality system supports compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a dry environment to maintain activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	Contact for details (Units/mg protein)
Purity (SDS-PAGE)	≥ 90%
Protein Content	Determined by COA
Stability	Retains specified activity when stored as directed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.