Description

Hydroxyindole o-Methyltransferase is a specialized enzyme critical in the biosynthesis of melatonin and other methoxylated indole derivatives. This biocatalyst is essential for researchers and manufacturers requiring precise enzymatic methylation in metabolic pathway studies and fine chemical synthesis. It is primarily utilized in pharmaceutical research, biochemical production, and advanced diagnostic development, serving the needs of the life sciences and biotechnology sectors.

Application

• Pharmaceutical Research & Development: Key reagent for studying melatonin synthesis pathways, neurotransmitter metabolism, and chronobiology.
• Biocatalytic Synthesis: Used as a catalyst for the regioselective O-methylation of hydroxyindoles in the production of high-value fine chemicals and pharmaceutical intermediates.
• Enzyme Mechanism Studies: Essential for investigating methyltransferase enzyme kinetics, structure-function relationships, and inhibitor screening.
• Diagnostic Kit Manufacturing: Incorporated into enzymatic assays for clinical research and diagnostic applications related to hormonal and neurological disorders.
• Academic & Industrial Biotechnology: A fundamental tool for metabolic engineering and the development of novel biosynthetic routes for natural products.

Basic Information

Product Name	Hydroxyindole o-Methyltransferase
CAS No.	9029-77-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	HIOMT; Acetylserotonin O-methyltransferase; ASMT; Hydroxyindole O-methyltransferase; Melatonin Synthase; S-Adenosyl-L-methionine:acetylserotonin O-methyltransferase; EC 2.1.1.4
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for enzymatic activity and purity. Each batch is characterized for specific activity and protein content. Certificates of Analysis (COA) detailing assay results, purity, and stability are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This material is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant to maintain activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Protein Content (Biuret)	≥ [Value] %
Purity (SDS-PAGE)	≥ [Value] %
pH (in solution)	7.0 - 7.5
Endotoxin (LAL)	< [Value] EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.