Description

5-10-Methylenetetrahydrofolate Dehydrogenase Type I is a critical enzyme involved in the folate metabolic pathway, specifically catalyzing the reversible conversion of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate. This enzymatic activity is essential for cellular one-carbon metabolism, impacting nucleotide synthesis and amino acid homeostasis. It is a vital reagent for biochemical research, pharmaceutical development, and diagnostic applications where precise folate cycle analysis is required.

Application

• Biochemical Research: Used as a key enzyme in studies of folate metabolism, one-carbon transfer mechanisms, and related metabolic pathways.
• Pharmaceutical Development: Employed in target validation, screening assays, and mechanism-of-action studies for drugs targeting folate-dependent processes, such as antifolate chemotherapeutics.
• Diagnostic Reagent Manufacturing: Serves as a component in enzymatic assay kits for the quantification of folate derivatives or related metabolites in clinical and research laboratories.
• Enzyme Kinetics Studies: Utilized to investigate enzyme kinetics, inhibition, and regulation under various experimental conditions.
• Metabolic Pathway Analysis: Applied in in vitro reconstructions of metabolic networks to study the regulation and flux of the folate cycle.
• Educational & Reference Standard: Used in academic settings for teaching advanced biochemistry and as a purified standard in analytical methods.

Basic Information

Product Name	5-10-Methylenetetrahydrofolate Dehydrogenase Type I
CAS No.	9029-14-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5,10-Methylenetetrahydrofolate Dehydrogenase (NADP+); 5,10-Methylenetetrahydrofolate:NADP+ Oxidoreductase; MTHFD1; C1-THF Synthase (Dehydrogenase Component); NADP-dependent Methylenetetrahydrofolate Dehydrogenase; EC 1.5.1.5; 5,10-CH2-THF Dehydrogenase; Methylenetetrahydrofolate Dehydrogenase (NADP+)
EINECS	Contact for details

Quality Control

Our 5-10-Methylenetetrahydrofolate Dehydrogenase Type I is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. Quality parameters typically assessed include protein concentration, specific activity, and absence of contaminating proteases or nucleases. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption, which can affect enzymatic activity.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Protein Content (Biuret)	>90%
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 95%
pH (in solution)	7.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/μg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.