Description

L-Amino Acid Dehydrogenase is a highly specific oxidoreductase enzyme that catalyzes the reversible oxidative deamination of L-amino acids to their corresponding α-keto acids, utilizing NAD+ or NADP+ as a cofactor. This biocatalyst is essential for precise biochemical analysis and synthesis, offering superior stereoselectivity and efficiency over chemical methods. It is a critical reagent for pharmaceutical R&D, diagnostic kit manufacturing, and academic research in enzymology and metabolic studies.

Application

• Diagnostic Assays: Key component in clinical chemistry for the quantitative determination of specific L-amino acids (e.g., L-Leucine, L-Phenylalanine) in blood and serum samples.
• Pharmaceutical Synthesis: Used in the enzymatic production of chiral intermediates and active pharmaceutical ingredients (APIs) requiring high enantiomeric purity.
• Biochemical Research: Essential tool for studying amino acid metabolism, enzyme kinetics, and metabolic pathway engineering in vitro.
• Food & Beverage Analysis: Employed in enzymatic methods for the accurate quantification of free amino acids in food products and fermentation broths.
• Biosensor Development: Integrated into enzyme-based electrodes and probes for real-time, selective monitoring of amino acid levels.
• Quality Control: Used in biotechnology and pharmaceutical QC labs to assay amino acid content in raw materials and finished products.

Basic Information

Product Name	L-Amino Acid Dehydrogenase
CAS No.	9029-13-4
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and subunit composition
Synonyms	L-Amino-Acid Dehydrogenase; NAD(P)+-L-Amino-Acid Dehydrogenase; L-Amino Acid:NAD+ Oxidoreductase (deaminating); EC 1.4.1.5; L-AADH; Leucine Dehydrogenase (common specific type); L-Amino Acid Oxidoreductase
EINECS	Contact for details

Quality Control

Our L-Amino Acid Dehydrogenase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity (via SDS-PAGE), and stability to ensure consistent performance. A comprehensive Certificate of Analysis (COA) detailing activity units/mg protein, protein concentration, pH optimum, and specific cofactor requirement (NAD+ or NADP+) is provided with every shipment. We support compliance with relevant guidelines for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted enzyme solutions should be aliquoted and stored at -20°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly in a dry environment after each use.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ [Value] units/mg protein
Protein Content	By Biuret or Bradford method
Purity (SDS-PAGE)	≥ 90%
pH Optimum	8.0 - 10.0 (varies by source)
Cofactor Specificity	NAD+ or NADP+ (as specified)
Stability	Retains ≥ 90% activity after 12 months at -20°C
Solubility	Soluble in buffer or water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.