Description

Ec 1.4.1.3 CAS NO 9029-11-2 is a high-purity enzyme preparation, specifically L-glutamate dehydrogenase (GDH). This product is essential for biochemical research and diagnostic applications, serving as a critical reagent in enzymatic assays and biosensor development. It is primarily utilized by biotechnology firms, diagnostic kit manufacturers, and research institutions in life sciences.

Application

• Diagnostic Reagent Manufacturing: A key component in clinical chemistry analyzers for the quantitative determination of blood urea nitrogen (BUN) and ammonia levels.
• Biochemical Research: Used as a model enzyme in studies of dehydrogenase kinetics, protein structure-function relationships, and nitrogen metabolism pathways.
• Biosensor Development: Integrated into amperometric and optical biosensors for the detection of glutamate, ammonia, and related metabolites in environmental and food analysis.
• Pharmaceutical Quality Control: Employed in enzymatic methods for the assay of specific amino acids and related compounds in drug substances and products.
• Food & Beverage Analysis: Applied in enzymatic test kits to monitor glutamate content, a key flavor enhancer, and to assess fermentation processes.
• Industrial Biotechnology: Potential use in biocatalytic processes for the synthesis of chiral intermediates or in metabolic engineering.

Basic Information

Product Name	Ec 1.4.1.3 (L-Glutamate Dehydrogenase)
CAS No.	9029-11-2
Molecular Formula	Protein (Enzyme)
Molecular Weight	~330,000 Da (Hexamer, species-dependent)
Synonyms	L-Glutamate Dehydrogenase; GDH; Glutamic Dehydrogenase; NAD(P)+-dependent Glutamate Dehydrogenase; EC 1.4.1.3; L-Glutamate:NAD(P)+ Oxidoreductase (Deaminating); Glutamate Dehydrogenase (NAD(P)+); Bovine Liver GDH (common source); Glutamate Dehydrogenase from Bovine Liver.
EINECS	Contact for details

Quality Control

Our L-Glutamate Dehydrogenase is produced and tested under a quality management system to ensure batch-to-batch consistency and performance. Each lot undergoes rigorous analytical testing, including activity assay, purity assessment (SDS-PAGE), and stability checks. A comprehensive Certificate of Analysis (COA) detailing specific activity (U/mg protein), protein concentration, and purity is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at 2-8°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to prevent activity loss. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use to avoid moisture uptake.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Identification (Activity)	Conforms
Specific Activity	≥ 40 units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
pH (in solution)	7.0 - 7.5
Stability	Stable for 24 months at 2-8°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.