Description

L-Alanine Dehydrogenase is a highly specific oxidoreductase enzyme that catalyzes the reversible deamination of L-alanine to pyruvate. This reaction is critical in nitrogen metabolism and biosynthetic pathways, making the enzyme a valuable tool for biochemical research and diagnostic applications. It is essential for laboratories and industries focused on amino acid analysis, metabolic studies, and the development of enzymatic assay kits.

Application

• Biochemical Research & Assay Development: Used as a key reagent in enzymatic assays for the quantitative determination of L-alanine or pyruvate in biological samples.
• Diagnostic Kit Manufacturing: Incorporated into clinical diagnostic kits for measuring alanine aminotransferase (ALT) activity, a crucial liver function test.
• Metabolic Pathway Studies: Employed in vitro to study the alanine cycle, gluconeogenesis, and other nitrogen metabolism pathways.
• Enzyme Engineering & Characterization: Serves as a model enzyme for studies on dehydrogenase kinetics, substrate specificity, and protein stability.
• Food & Beverage Analysis: Utilized in enzymatic methods for the analysis of free amino acid content in food products and fermentation broths.
• Pharmaceutical Intermediate Synthesis: Applied in biocatalytic processes for the stereospecific synthesis of chiral amino acids and related compounds.

Basic Information

Product Name	L-Alanine Dehydrogenase
CAS No.	9029-06-5
Molecular Formula	N/A (Enzyme)
Molecular Weight	~200,000 Da (Approx., varies by source)
Synonyms	L-Alanine:NAD+ Oxidoreductase (deaminating); Alanine Dehydrogenase; L-AlaDH; NAD+-L-Alanine Dehydrogenase; EC 1.4.1.1; L-Alanine NAD Oxidoreductase
EINECS	Contact for details

Quality Control

Our L-Alanine Dehydrogenase is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity, and stability to ensure consistent performance in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing activity units, protein content, and impurity profiles is provided with every shipment to guarantee reliable and reproducible results for our clients.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to prevent loss of activity. The product is hygroscopic (moisture-sensitive); always allow the vial to equilibrate to room temperature before opening to avoid condensation.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Specific Activity	≥ 50 units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
Stabilizers	Potassium phosphate, EDTA, Glycerol
pH (in solution)	7.0 - 7.5
Optimum pH	9.0 - 10.5
Optimum Temperature	25 - 37°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.