Description

Choline Oxidase is a highly specific oxidoreductase enzyme that catalyzes the oxidation of choline to betaine aldehyde, with the concomitant production of hydrogen peroxide. This precise catalytic activity makes it an indispensable reagent for enzymatic assays, diagnostic kits, and biochemical research where the detection or quantification of choline is critical. It is essential for manufacturers and researchers in the in-vitro diagnostics (IVD), pharmaceutical quality control, food analysis, and life science research sectors.

Application

• Clinical Diagnostics & IVD Kits: Core component in enzymatic colorimetric and electrochemical assays for the quantitative determination of choline in blood, plasma, and other biological fluids.
• Pharmaceutical Analysis: Used in enzymatic methods for the assay of choline-containing active pharmaceutical ingredients (APIs) and in stability-indicating studies.
• Food & Nutritional Analysis: Employed in enzymatic kits to measure choline content in infant formula, dietary supplements, and fortified food products for nutritional labeling.
• Biochemical Research: A key tool for studying choline metabolism, phospholipid biosynthesis, and as a coupling enzyme in multi-enzyme reaction systems.
• Biosensor Development: Integrated into amperometric and optical biosensor platforms for real-time, selective detection of choline in various matrices.
• Microbiology: Used in studies of microbial pathways and for the identification of bacteria based on choline utilization.

Basic Information

Product Name	Choline Oxidase
CAS No.	9028-67-5
Molecular Formula	N/A (Enzyme)
Molecular Weight	Approx. 70-80 kDa (Monomer)
Synonyms	Choline:oxygen 1-oxidoreductase; 1.1.3.17; Choline Dehydrogenase (ambiguous); COD; ChO; Arthrobacter globiformis choline oxidase; Recombinant Choline Oxidase
EINECS	Contact for details

Quality Control

Our Choline Oxidase is produced and tested under a rigorous quality management system. Each lot is characterized for critical parameters including specific activity, purity (SDS-PAGE), and stability to ensure batch-to-batch consistency and performance reliability for your diagnostic and analytical applications. A comprehensive Certificate of Analysis (COA) detailing activity units, protein content, and storage buffer is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C. Reconstituted enzyme solutions should be aliquoted and stored at -20°C or below to minimize freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Conforms
Specific Activity	≥ 10 U/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	≥ 90%
pH Optimum	~8.0
Stability	≥ 90% activity after 12 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.