Description

Ec 1.1.1.42 is a high-purity enzyme preparation, also known as Isocitrate Dehydrogenase (NADP+), with the CAS registry number 9028-48-2. This biocatalyst is essential for research and industrial processes involving the citric acid cycle, specifically catalyzing the oxidative decarboxylation of isocitrate to α-ketoglutarate. It is a critical reagent for biochemical research, diagnostic kit manufacturing, and enzymatic synthesis. Industries requiring precise metabolic pathway analysis or cofactor regeneration systems rely on its consistent activity and purity.

Application

• Biochemical Research & Assay Development: A key component in enzymatic assays for measuring isocitrate, NADP+, or metal ions like Mg²⁺/Mn²⁺.
• Diagnostic Kit Manufacturing: Used in clinical chemistry kits for the determination of substrates or enzymes in metabolic panels.
• Enzymatic Synthesis: Employed in vitro for the stereospecific production of α-ketoglutarate or related intermediates.
• Metabolic Pathway Studies: Essential for in vitro reconstructions of the Krebs cycle and studies on cellular energy metabolism.
• Pharmaceutical Screening: Utilized as a target enzyme in high-throughput screening for potential metabolic disorder therapeutics.
• Educational & Laboratory Use: Serves as a standard enzyme for teaching biochemistry principles and laboratory techniques.

Basic Information

Product Name	Ec 1.1.1.42
CAS No.	9028-48-2
Molecular Formula	N/A (Enzyme)
Molecular Weight	Varies by source and subunit composition. Contact for details.
Synonyms	Isocitrate Dehydrogenase (NADP+); NADP+-specific ICDH; IDH; IDP; Oxalosuccinate decarboxylase; Three-DS-Isocitrate:NADP+ oxidoreductase (decarboxylating); EC 1.1.1.42
EINECS	Contact for details.

Quality Control

Our Ec 1.1.1.42 is produced and tested under stringent quality management systems. Each lot is characterized for specific activity, purity (via SDS-PAGE), and stability to ensure performance consistency for critical applications. Certificates of Analysis (COA) detailing assay results, protein concentration, and storage buffer composition are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Store in a dry environment.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution or lyophilized powder
Identification (Activity)	Conforms (Specific activity provided on COA)
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	Provided on COA
Stabilizers/Buffer	Potassium phosphate, glycerol, DTT (typical)
pH	7.0 - 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.