Description

D-3-Hydroxybutyrate Dehydrogenase is a high-purity enzyme catalyst essential for the specific oxidation of D-3-hydroxybutyrate to acetoacetate. This specificity makes it a critical reagent for enzymatic assays and diagnostic kits, ensuring accurate measurement of ketone body levels. It is primarily required by manufacturers in the in-vitro diagnostic (IVD), pharmaceutical research, and biochemical analysis sectors for developing reliable testing solutions.

Application

• Clinical Diagnostics: Key component in enzymatic assay kits for the quantitative determination of 3-hydroxybutyrate (ketone bodies) in blood, serum, or urine, crucial for managing diabetic ketoacidosis.
• Biochemical Research: Used as a specific enzyme in metabolic pathway studies, particularly in research focused on ketone body metabolism, fatty acid oxidation, and energy homeostasis.
• Pharmaceutical Development: Employed in enzyme-coupled reactions for screening potential drug candidates that modulate ketogenesis or related metabolic disorders.
• Food & Beverage Analysis: Applied in enzymatic methods for the detection and quantification of specific organic acids or metabolites in quality control laboratories.
• Enzyme Engineering: Serves as a model enzyme for studies on dehydrogenase activity, stability, and immobilization techniques for biosensor development.

Basic Information

Product Name	D-3-Hydroxybutyrate Dehydrogenase
CAS No.	9028-38-0
Molecular Formula	N/A (Enzyme)
Molecular Weight	Approx. 31,000 Da (monomer) - Contact for details
Synonyms	3-Hydroxybutyrate Dehydrogenase; D-β-Hydroxybutyrate Dehydrogenase; HBDH; D-3-Hydroxybutyric Dehydrogenase; (R)-3-Hydroxybutyrate Dehydrogenase; 3-Hydroxybutyrate:NAD+ Oxidoreductase; EC 1.1.1.30
EINECS	Contact for details

Quality Control

Our D-3-Hydroxybutyrate Dehydrogenase is produced and tested under stringent quality management systems. Each batch is characterized for specific activity, purity (via SDS-PAGE), and stability to ensure consistent performance in sensitive diagnostic applications. Certificates of Analysis (COA) detailing activity units, protein content, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Catalyzes the specific oxidation of D-3-hydroxybutyrate
Specific Activity	> 100 U/mg protein (Contact for specific grade)
Purity (SDS-PAGE)	> 90%
Protein Content (Biuret)	Provided on COA
Stabilizers / Buffers	May contain potassium phosphate, glycerol
Microbial Limit	Meets specifications for enzyme reagents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.