Description

Ec 1.1.1.28 CAS NO 9028-36-8 is a high-purity enzyme preparation, specifically L-Lactate Dehydrogenase (LDH), which is critical for catalyzing the interconversion of lactate and pyruvate with concomitant interconversion of NADH and NAD+. This biocatalyst is essential for reliable and reproducible results in diagnostic, research, and industrial enzymatic assays. It is primarily utilized by manufacturers and research institutions in the in-vitro diagnostics (IVD), life science research, and bioprocessing sectors.

Application

• Clinical Diagnostics: A key component in enzymatic assay kits for the quantitative determination of lactate, pyruvate, and related metabolites in blood and other biological fluids.
• Biochemical Research: Used as a critical reagent in enzyme kinetics studies, metabolic pathway analysis, and cellular respiration research.
• Food & Beverage Analysis: Employed in enzymatic methods for the accurate measurement of L-lactate and D-lactate in food quality control and fermentation process monitoring.
• Pharmaceutical Manufacturing: Serves as a tool in quality control for monitoring fermentation processes and ensuring batch consistency in biopharmaceutical production.
• Cell Culture & Cytotoxicity Assays: Used to measure lactate dehydrogenase activity released from cells as a standard marker for cell viability, proliferation, and cytotoxicity (e.g., in drug screening).
• Veterinary Diagnostics: Applied in veterinary medicine for metabolic profiling and disease diagnosis in livestock and companion animals.

Basic Information

Product Name	Ec 1.1.1.28 CAS NO 9028-36-8
CAS No.	9028-36-8
Molecular Formula	Protein (Enzyme)
Molecular Weight	~140,000 Da (Tetramer)
Synonyms	L-Lactate Dehydrogenase; LDH; Lactate Dehydrogenase (L-Lactate); L-Lactate:NAD+ Oxidoreductase; 1.1.1.27 (common mis-association); Heart LDH; Muscle LDH; NAD-dependent lactate dehydrogenase
EINECS	Contact for details

Quality Control

Our L-Lactate Dehydrogenase is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing parameters such as specific activity, protein content, and contaminant levels. Production can adhere to relevant guidelines for in-vitro diagnostic (IVD) and research use only (RUO) applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. The product is hygroscopic (moisture-sensitive). Avoid repeated freeze-thaw cycles. Under these conditions, the product is stable for the duration indicated on the label.

Specification

Item	Specification
Appearance	Lyophilized powder or suspension
Identification (IR/Activity)	Conforms
Specific Activity	> 300 units/mg protein
Protein Content (Biuret)	Contact for details
Purity (SDS-PAGE)	> 95%
Solubility	Clear solution in buffer
pH (in solution)	6.0 - 7.5
Stabilizers/Additives	May contain buffer salts (e.g., phosphate, sulfate)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.